盐酸四环素长期暴露对序批式反应器性能、微生物酶活性及微生物群落的影响  

Long-Term Exposure Effect of Tetracycline on Performance,Microbial Enzyme Activity and Microbial Community in Sequencing Batch Reactor

作　　者：齐维毅 王倩芝 楚光玉 陈文正 张雨乔 刘甲滕 高畅 鲁帅领 高孟春[1,2] Qi Weiyi;Wang Qianzhi;Chu Guangyu;Chen Wenzheng;Zhang Yuqiao;Liu Jiateng;Gao Chang;Lu Shuailing;Gao Mengchun(The Key Laboratory of Marine Environmental Science and Ecology,Ministry of Education,Qingdao 266100,China;College of Environmental Science and Engineering,Ocean University of China,Qingdao 266100,China)

机构地区：[1]中国海洋大学海洋环境与生态教育部重点实验室,山东青岛266100 [2]中国海洋大学环境科学与工程学院,山东青岛266100

出　　处：《中国海洋大学学报（自然科学版）》2024年第3期88-98,共11页Periodical of Ocean University of China

基　　金：中央高校基本科研业务费专项(201964003)资助。

摘　　要：本文研究了0.5 mg/L盐酸四环素(TC)长期暴露对序批式反应器(SBR)性能、脱氮酶活性、抗氧化酶和微生物群落的影响。结果表明,SBR中有机物去除能力和氨氧化能力随运行时间的延长而降低,在反应器运行第70天时,COD去除率从97.10%降低至70.33%,NH_(4)^(+)-N去除率从99.24%降低至67.44%,出水NO_(3)^(-)-N逐渐降低,SBR内没有出现NO_(2)^(-)-N积累。TC长期暴露降低了氨单加氧酶(AMO)、亚硝酸盐氧化酶(NOR)、硝酸盐还原酶(NR)和亚硝酸盐还原酶(NIR)等脱氮酶活性,增加了活性氧(ROS)的相对产生量、乳酸脱氢酶(LDH)相对释放量和部分抗氧化酶的相对活性。进水中加入TC后,微生物的丰富度、多样性和均匀性均降低,硝化菌属(Nitrospira)和反硝化菌属(Zoogloea、Thauera、Dechloromonas、Dokdonella、Hyphomicrobium和Pseudomonas)的相对丰度发生变化,微生物群落和关键微生物具有显著性差异,从而影响了SBR的脱氮性能。The performance,microbial enzymatic activity,antioxidant enzymes and microbial community of a sequencing batch reactor(SBR)have been evaluated under long-term exposure of tetracycline(TC).The results showed that the COD removal efficiency and NH_(4)^(+)-N removal efficiency of SBR decreased with the increase of operation time.On day 70,COD removal efficiency decreased from 97.10%to 70.33%,NH_(4)^(+)-N removal efficiency decreased from 99.24%to 67.44%,and NO_(3)^(-)-N in the effluent decreased gradually,and NO_(2)^(-)-N accumulation did not occur in SBR.Long-term exposure of TC decreased the activities of denitrogenase(AMO),nitrite oxidase(NOR),nitrate reductase(NR)and nitrite reductase(NIR),and increased the relative production of reactive oxygen species(ROS),the relative release of lactate dehydrogenase(LDH)and the relative activities of some antioxidant enzymes.Under the pressure of TC,the richness,diversity and uniformity of microorganisms decreased.The relative abundance of Nitrospira and denitrifiers(Zoogloea,Thauera,Dechloromonas,Dokdonella,Hyphomicrobium and Pseudomonas)changed.There were significant differences between the microbial community and keystone taxa,which affected the nitrogen removal performance of SBR.

关 键 词：盐酸四环素 微生物酶活性 微生物群落 LEfSe分析 关键微生物 

分 类 号：X55[环境科学与工程—环境工程]