一株与沙门菌血清产生交叉凝集的奇异变形杆菌检验  

Detection of a strain of proteus mirabilis that produced cross-agglutinating with Salmonella sera

作　　者：王达伟 张辉 李谨 WANG Da-wei;ZHANG Hui;LI Jin(Tianjin Railway Center for Disease Control and Prevention,China Railway Beijing Bureau Group Co.,Ltd.,Tianjin 300140,China;School of Engineering and Technology,Tianjin Agricultural University)

机构地区：[1]中国铁路北京局集团有限公司天津铁路疾病预防控制中心,天津300140 [2]天津农学院工程技术学院

出　　处：《中国城乡企业卫生》2023年第12期53-55,共3页Chinese Journal of Urban and Rural Enterprise Hygiene

摘　　要：目的为了提高实验室病原微生物检测能力,提升应对突发公共卫生事件的应急水平。中国铁路北京局集团有限公司天津铁路疾病预防控制中心于2019年7月参加辽宁检验检疫局检验中心下发的食品中微生物检测能力验证室间质控考核,对样本进行检验。方法依据实验室质控考核样本说明,同时参照4789.4-2016《食品安全国家标准食品微生物学检验沙门氏菌检验》对编号为S166的食源性致病菌样本进行前处理、增菌培养、分离鉴定、血清凝集实验等最终通过实验室生化鉴定系统出具结果报告。结果在送检样品中没有检测出沙门菌,但是分离培养出一株靛基质生化反应阳性且与沙门菌多价血清A-F-O产生交叉凝集的奇异变形杆菌。结论沙门菌检测中,发现初步生化鉴定符合沙门菌多价血清A-F-O凝集的菌株,应进一步深入鉴定,不可盲目判定结果。除了结合菌落形态特点外应主要依据生化鉴定及血清凝集实验结果综合鉴定。此次考核也为预防沙门菌误检的发生及沙门菌的流行提供理论依据及技术支持。Objective In order to improve the detection ability of pathogenic microorganisms in the laboratory,and improve the emergency level of responding to public health emergencies.Tianjin Railway Center for Disease Control and Prevention,China Railway Beijing Bureau Group Co.,Ltd.,participated in the inter-laboratory quality control assessment of microbial detection capability verification in food issued by the Inspection Center of Liaoning Inspection and Quarantine Bureau to detection the samples in July 2019.Methods The food-borne pathogen samples numbered S166 were pre-treated,enrichment cultured,isolated and identified,serum agglutination experiments,etc.,according to the laboratory quality control assessment sample statement and with reference to 4789.4-2016 National Food Safety Standard Food Microbiological Inspection Salmonella Inspection,finally the results report was issued through the laboratory biochemical identification system.Results No Salmonella was detected in the samples submitted for inspection,but a strain of proteus mirabilis with positive biochemical reaction of indigo matrix and crossagglutination with Salmonella polyvalent serum A-F-O was isolated and cultured.Conclusion In the Salmonella detection,the strains of agglutinated with salmonella polyvalent serum A-F-O was found in the preliminary biochemical identification which should be further identified,and the results should not be judged blindly.In addition to combining the morphological characteristics of the colony,it should be mainly based on the comprehensive identification of biochemical identification and serum agglutination test results.This assessment also provided theoretical basis and technical support for preventing the occurrence of false detection and prevalence of Salmonella.

关 键 词：沙门菌 生化鉴定 盲样 

分 类 号：R4[医药卫生—临床医学]