不同培养条件对小麦根际可培养微生物影响  

Effects of different cultivation conditions on cultivable microorganisms in wheat rhizosphere

作　　者：吕路琼 欧阳由男[2] 叶淑珍 游雨欣 李斌[3] 王艳丽[1] LYU Luqiong;OUYANG Younan;YE Shuzhen;YOU Yuxin;LI Bin;WANG Yanli(Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences,State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products,Zhejiang Provincial National Crop Variety Resistance Identification Test Station,Hangzhou 310021,Zhejiang;China National Rice Research Institute,Hangzhou 310006,Zhejiang;Institute of Biotechnology Research,Zhejiang University,Hangzhou 310058,Zhejiang)

机构地区：[1]浙江省农业科学院植物保护与微生物研究所,省部共建农产品质量安全危害因子与风险防控国家重点实验室,浙江省国家农作物品种抗性鉴定试验站,浙江杭州310021 [2]中国水稻研究所,浙江杭州310006 [3]浙江大学生物技术研究所,浙江杭州310058

出　　处：《浙江农业科学》2024年第2期287-291,共5页Journal of Zhejiang Agricultural Sciences

基　　金：杭州市科技发展计划项目(202003A05);浙江省农业科学院省部共建农产品质量安全危害因子与风险防控国家重点实验室自主设计项目(2010DS700124-ZZ2014)。

摘　　要：植物根际微生物群落对植物健康生长有重要影响,分离培养其微生物对农作物促生增产具有重要意义。在微生物培养过程中,培养基类型与充氧量对根际微生物富集情况不同。该研究采用16S rRNA高通量测序技术与生物信息学分析对使用不同培养条件小麦根际细菌群落特征与功能进行分析。研究表明,小麦根际微生物群落主要由γ-变形菌纲,拟杆菌纲和芽孢杆菌纲组成,且培养基类型和充氧量显著影响其丰度。FAPROTAX功能预测表明,小麦根际微生物富含的优势菌群主要与化能异养、发酵、碳循环、氮循环、铁呼吸等功能相关,对植物生长发育具有重要作用。研究结果为小麦根际微生物培养及微生物资源的开发利用提供重要依据。The rhizosphere microbial community of plants has a significant impact on their healthy growth,and isolating and cultivating their microorganisms is of great significance for promoting crop growth and yield increase.During the process of microbial cultivation,the type of culture medium and oxygenation capacity have different effects on the enrichment of rhizosphere microorganisms.This study used 16S rRNA high-throughput sequencing technology and bioinformatics analysis to analyze the characteristics and functions of wheat rhizosphere bacterial communities under different cultivation conditions.Research has shown that the microbial community in the rhizosphere of wheat is mainly composed of γ-Proteobacteria,Bacteroides,and Bacillus,and the type of culture medium and oxygen content significantly affect their abundance.The functional prediction of FAPROTAX indicated that the dominant microbial community in wheat rhizosphere microorganisms is mainly related to functions such as chemical heterotrophic,fermentation,carbon cycling,nitrogen cycling,and iron respiration,which played an important role in plant growth and development.The research results provided important basis for the cultivation of wheat rhizosphere microorganisms and the development and utilization of microbial resources.

关 键 词：根际微生物 培养基 充氧量 高通量测序 菌群特征 

分 类 号：S432.4[农业科学—植物病理学]