HPLC法测定参梅养胃颗粒(无蔗糖)中羟基红花黄色素A的含量  

Determination of Hydroxysafflower Yellow A in Shenmei Yangwei Granules (Without Sucrose) by HPLC

作　　者：路丽 张洪坤 黄玉瑶 郝建新 仲勇成 王利胜[4] 董翼虎 高贯彪 LU Li;ZHANG Hongkun;HUANG Yuyao;HAO Jianxin;ZHONG Yongcheng;WANG Lisheng;DONG Yihu;GAO Guanbiao(Sichuan Haoyun Pharmaceutical Co.,Ltd.,Guangyuan 628017,China;Bozhou Huqiao pharmaceutical industy limited company,Bozhou 236800,China;Guangzhou Heyi Medical Technology Co.,Ltd.,Guangzhou 510970,China;Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Guangyuan Drug Inspection and Testing Center,Guangyuan 628017,China)

机构地区：[1]四川豪运药业股份有限公司,四川广元628017 [2]安徽省亳州市沪谯药业有限公司,安徽亳州236800 [3]广州市和一医疗科技有限公司,广东广州510970 [4]广州中医药大学,广东广州510006 [5]四川省广元市药品检验检测中心,四川广元628017

出　　处：《中国民族民间医药》2024年第3期41-45,共5页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:建立HPLC法测定参梅养胃颗粒(无蔗糖)中羟基红花黄色素A的含量。方法:采用色谱柱ODS-C_(18)(4.6 mm×250 mm, 5μm);以甲醇-乙腈-0.7%磷酸溶液(26∶2∶72)为流动相等度洗脱;检测波长403 nm;柱温25℃;进样量10μL;流速1.0 mL/min。结果:羟基红花黄色素A在0.0480~0.7196μg范围内线性关系良好,平均加样回收率(n=9)为101.74%, RSD值为1.6%。结论:该方法操作简便、准确、重复性好,测定结果稳定,可以作为参梅养胃颗粒(无蔗糖)中羟基红花黄色素A的含量测定方法。Objective To establish an HPLC method for the determination of hydroxysafflower yellow A in Shenmei Yangwei granules(without sucrose).Methods Chromatographic column ODS-C_(18)(4.6mm × 250mm, 5 μm), elute with methanol-acetonitrile-0.7% phosphoric acid solution(26:2:72) as the flow equivalent, detection wavelength: 403 nm, column temperature 25℃,injection volume 10 μL, the flow rate is 1.0 mL/min.Results Hydroxysafflower yellow A was 0.0480-0.7196 μg the linear relationship within the range of is good, the average recovery rate(n=9) is 101.74%, and the RSD value is 1.6%.Conclusion The method is simple, accurate, reproducible and stable. It can be used for the determination of hydroxysafflower yellow A in Shenmei Yangwei granules(without sucrose).

关 键 词：参梅养胃颗粒(无蔗糖) 羟基红花黄色素A 含量测定 HPLC法 

分 类 号：R284.1[医药卫生—中药学]