高透光性甜菜碱型明胶水凝胶的制备与表征  

Preparation and characterization of betaine-type gelatinhydrogels with high light transmittance

作　　者：赵丽琴 贾晋一 许慧 张秀梅 彭秀枝 魏延 黄棣 ZHAO Liqin;JIA Jinyi;XU Hui;ZHANG Xiumei;PENG Xiuzhi;WEI Yan;HUANG Di(Research Center for Nano-Biomaterials&Regenerative Medicine,Department of Biomedical Engineering,College of Biomedical Engineering,Taiyuan University of Technology,Taiyuan 030024,China;Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering,Taiyuan 030032,China)

机构地区：[1]太原理工大学生物医学工程学院生物医学工程系,纳米生物材料与再生医学研究中心,太原030024 [2]山西浙大新材料与化工研究院,太原030032

出　　处：《功能材料》2024年第1期1025-1030,共6页Journal of Functional Materials

基　　金：国家自然科学基金项目(82103147,12252273);山西省自然科学基金项目(20210302124405)。

摘　　要：甲基丙烯酰化明胶(gelatin methacryloyl,GelMA)水凝胶透光性、抗蛋白吸附能力较弱,限制了其在人工角膜的应用。基于GelMA水凝胶,引入具抗生物吸附能力及生物相容性的磺基甜菜碱甲基丙烯酸酯(Methacryloxyethyl sulfobetaine,SBMA),构建系列比例的复合两性离子水凝胶GelMA-SBMA体系,讨论单体配比对水凝胶理化性能、生物学性能的影响,并进行体外细胞实验,讨论复合水凝胶对细胞生长、增殖的影响。结果表明,GelMA∶SBMA为5∶1的复合水凝胶在700 nm波长下透光率可达94%,与纤维蛋白的接触角保持在90°以上,体现出抗蛋白吸附能力。细胞实验表明,SBMA的引入有助于细胞生长、增殖,体现出良好的生物相容性。Due to the poor light transmission,anti-protein adsorption capacity and flexibility,methacryloylated gelatin(GelMA)hydrogel is limited in artificial cornea.This paper aims to introduce methacryloxyethyl sulfobetaine(SBMA)with excellent bioadsorption resistance and outstanding biocompatibility to GelMA hydrogel,and construct a series of zwitterionic hydrogel GelMA-SBMA system.The physicochemical and biological properties of hydrogels are discussed,and the vitro cell experiments are conducted to discuss the effects of composite hydrogels on cell growth and proliferation.The results show that GelMA-SBMA hydrogel in ratio of 5∶1,at a wavelength of 700 nm,presents a light transmittance of 94%.The good anti-protein adsorption ability is reflected by the contact angle with fibrin above 90°.The vitro cell experiments show that SBMA is more conducive to cell growth and proliferation with good biocompatibility.

关 键 词：透光性 甲基丙烯酰化明胶 磺基甜菜碱甲基丙烯酸酯 生物相容性 抗蛋白吸附 

分 类 号：TB332[一般工业技术—材料科学与工程] TB34