潞党参根腐病根际土壤真菌群落及与土壤因子的关系  

Fungal Community in Rhizosphere Soil of Root Rot of Lu Dangshen(Codonopsis pilosula)and Its Relationship with Soil Factors

作　　者：刘晓丽 张月荣 姜霞 杜晨晖 詹海仙 Liu Xiaoli;Zhang Yuerong;Jiang Xia;Du Chenhui;Zhan Haixian(School of Chinese Materia Medica and Food Engineering,Shanxi University of Chinese Medicine,Jinzhong,030619)

机构地区：[1]山西中医药大学中药与食品工程学院,晋中030619

出　　处：《分子植物育种》2024年第4期1233-1241,共9页Molecular Plant Breeding

基　　金：山西重点研发计划项目(201903D221053);山西省现代农业产业技术体系建设专项(2011-11);山西省中医药管理局科研课题(2022ZYYC096)共同资助。

摘　　要：为了解潞党参根腐病发病前后根际土壤真菌的群落多样性及其与土壤因子的关系,本研究采用高通量测序技术对根腐病潞党参和健康植株根际土壤中的真菌微生物群落构成进行分析。结果表明,潞党参根际真菌群落丰度的降低与潞党参根腐病发生具有一定的相关性,不同分类水平潞党参病健植株根际真菌组成和某些优势菌群的丰度在两组之间均存在差异。白粉菌属和拟青霉属只存在于健康潞党参植株土壤中,四枝孢属(Tetracladium)丰度在根腐病潞党参根际土壤中最高。冗余分析结果表明,QD(总氮)及YJZ(有机质)与枝孢菌属(Cladosporium)、镰刀菌属(Fusarium)和白粉菌属(Erysiphe)的含量呈正相关,SXJ(速效钾)对真菌群落分布的影响不具有显著性(P=0.082)。但其他各类差异菌群功能及其与潞党参根腐病病原菌的互作关系尚待研究。In order to understand the community diversity of fungi in rhizosphere soil and its relationship with environmental factors before and after the root rot of Lu Dangshen(Codonopsis pilosula),in this study,we used highthroughput sequencing technology to analyze the composition of fungal microbiomes in the rhizosphere soil of Lu Dangshen and healthy plants.The results showed that there was a certain correlation between the decrease of the abundance of the rhizosphere fungi community and the occurrence of the root rot of Lu Dangshen,and there were differences between the two groups in the composition of the rhizosphere fungi and the abundance of some dominant fungi in the healthy plants of Lu Dangshen at different classification levels.Erysiphe and Paecilomyces only existed in the soil of healthy Lu Dangshen plants,and the abundance of Tetracladium was the highest in the rhizosphere soil of Lu Dangshen with root rot.The results of redundancy analysis showed that QD(total nitrogen)and YJZ(organic matter)were positively correlated with the contents of Cladosporium,Fusarium and Erysiphe,while SXJ(available potassium)had no significant effect on the distribution of fungal communities(P=0.082).However,the function of other types of different flora and their interaction with the pathogenic bacteria of root rot of Lu Dangshen still need to be studied.

关 键 词：潞党参 高通量测序 根际土壤 真菌群落 环境因子 

分 类 号：S435.675[农业科学—农业昆虫与害虫防治]