正常足月新生儿出生3个月内肠道菌群的定植规律分析  

Analysis of intestinal flora colonization in normal term neonates within 3 months after birth

作　　者：张金铃 姜华 杨萌婷 马丽 雷光霞 杜欣欣 冯梅 余涵 王鉴 ZHANG Jinling;JIANG Hua;YANG Mengting;MA Li;LEI Guangxia;DU Xinxin;FENG Mei;YU Han;WANG Jian(School of Clinical Medicine,Guizhou Medical University,Guiyang 550000,Guizhou,China;Department of Pediatric Surgery,the Affiliated Hospital of Guizhou Medical University,Guiyang 550004,Guizhou,China;Department of Neonatology,the Affiliated Hospital of Guizhou Medical University,Guiyang 550004,Guizhou,China)

机构地区：[1]贵州医科大学临床医学院,贵州贵阳550000 [2]贵州医科大学附属医院小儿外科,贵州贵阳550004 [3]贵州医科大学附属医院新生儿科,贵州贵阳550004

出　　处：《贵州医科大学学报》2024年第1期109-114,共6页Journal of Guizhou Medical University

基　　金：贵州省科技厅基础研究计划(自然科学类)(黔科合基础-ZK[2023]一般350)。

摘　　要：目的 探讨正常足月新生儿出生3个月内肠道菌群的定植规律。方法 收集20例正常足月新生儿及其孕母的临床资料,取新生儿出生后第1天(C0)、第3天(C1)、第1周(C2)、第1个月(C3)及第3个月(C4)的粪便标本,采用Illumina NovaSeq PE250进行16S核糖体RNA(16S rRNA)V3+V4区测序,采用生物信息学分析肠道菌群的定植规律。结果 正常足月新生儿粪便中肠道菌群的扩增子序列变异体(ASV)数目、Shannon指数于C0时最高;正常足月新生儿出生3个月内的肠道菌群以变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、放线菌门(Actinobacteriota)、拟杆菌门(Bacteroidota)为优势菌门,Proteobacteria相对丰度随日龄由高变低再逐渐增高,Firmicutes与之相反;正常足月新生儿生3个月内优势菌群在属水平的演变依次为罗尔斯通菌属(Ralstonia)、链球菌属(Streptococcus)、双歧杆菌属(Bifidobacterium),随日龄增加,Bifidobacterium相对丰度逐渐升高,C4时达44.18%且为优势菌属。结论 正常足月新生儿生3个月内肠道菌群的多样性、组成结构及相对丰度出现变化。Objective To investigate the colonization of intestinal flora in normal full-termneonates within 3 months after birth.Methods Clinical data of 20 normal full-term neonates and their pregnant mothers,and fecal specimens were collected from the 1 st day(C0),3 rd day(C1),1 st week(C2),1 st month(C3),and 3 rd month(C4)after birth.Illumina NovaSeq PE250 was used to sequencing and bioinformatic analysis of 16S ribosomal RNA(16S rRNA)V3+V4 region.Results The number of amplicon sequence variants(ASV)and Shannon index of intestinal flora in normal term neonates were the highest at C0.The postnatal intestinal flora of normal full-term neonates within 3 months after birth was dominated by Proteobacteria,Firmicutes,Actinobacteriota,and Bacteroidota.The relative abundance of Proteobacteria changed from high to low and then gradually increased with age,whereas Firmicutes were the opposite.Within 3 months after birth,the dominant bacteria in normal term neonates were Ralstonia,Streptococcus and Bifidobacterium at the genus level.With the increase of age,the relative abundance of Bifidobacterium increased gradually,reaching 44.18%at C4,and Bifidobacterium was the dominant bacterium.Conclusion The changes take place in diversity,composition and relative abundance of intestinal flora of normal full-term neonates within 3 months after birth.

关 键 词：婴儿 新生 足月儿 肠道菌群 多样性 相对丰度 16S核糖体RNA 

分 类 号：R722.1[医药卫生—儿科]