气候变化背景下低温胁迫对三角梅叶绿素荧光参数及抗氧化体系的影响  

0176-11 Effects of Low-Temperature Stress on Chlorophyll Fluorescence Parameters and Antioxidant System of Bougainvillea in the Context of Climate Change

作　　者：毛轩雯 陆锦灏 阮心依 白晓文 刘宏缘 赵杭 林雅楠 蒋诗睿 宋扬 张宝欣 谭驭宇 刘鹏[1] MAO Xuanwen;LU Jinhao;RUAN Xinyi;BAI Xiaowen;LIU Hongyuan;ZHAO Hang;LIN Ya’nan;JIANG Shirui;SONG Yang;ZHANG Baoxin;TAN Yuyu;LIU Peng(Botany Laboratory,College of Life Sciences,Zhejiang Normal University,Jinhua,Zhejiang 321004,China)

机构地区：[1]浙江师范大学生命科学学院植物学实验室,浙江金华321004

出　　处：《水土保持学报》2024年第1期176-186,共11页Journal of Soil and Water Conservation

基　　金：国家自然科学基金项目(32001224,41461010)。

摘　　要：[目的]为探明气候变化背景下低温对三角梅生理生化表现及其对低温胁迫的内在适应机制。[方法]以具有强、中、弱耐寒能力的宫粉、热火、百变三角梅为材料,采用室内盆栽试验,低温处理7,14,21天,研究不同耐寒型三角梅光合系统和抗氧化体系在各周期下对低温胁迫的响应情况。[结果](1)低温处理期间,各品种初始荧光(F 0)均有所上升,其中宫粉三角梅变化最为显著,较第0天增长60.2%,最大荧光(F m)、PSII有效光化学电子产量(F v/F m)以及光合相对电子传递速率(ETR)则迅速降低,并于第21天时达到最低水平。三角梅的光化学猝灭系数(qP)均随着低温胁迫加剧而下跌,而热火三角梅与宫粉三角梅的非光化学猝灭系数(qN)大幅提高。(2)低温胁迫后各品种三角梅SOD、POD、CAT酶活性均显著下降,并随着胁迫时间的延长而加剧,即低温对三角梅抗氧化酶活性产生严重抑制,其中宫粉三角梅的SOD、POD、CAT酶活性均高于其余2个品种,可见宫粉具有强抗寒能力。(3)在AsA-GSH循环体系中,低温下各三角梅叶AsA含量与DHA含量均明显增加,21天时3个品种三角梅的GR活性分别较0天上升180.91%,175.97%,112.37%,且宫粉三角梅在21天时AsA/DHA比率、APX活性与DHAR活性显著提升。[结论]低温通过影响PSⅡ光化学反应中心而抑制植株的生长,三角梅可通过开启光合系统保护机制减少光抑制,此外还积累渗透调节物质,启动抗氧化系统产生应激调节作用,以清除自身体内快速增生的ROS减轻低温伤害。[Objective]This study is aimed to explore the physiological and biochemical manifestations of Bougainvillea spectabilis Willd.to low temperature and the intrinsic adaptation mechanism to cold stress in the context of climate change.[Methods]Taking Bougainvillea Paederia Foetida,Bougainvillea Flaming Sumba and Bougainvillea Duranta with strong,medium,and weak cold tolerance as test materials,through indoor pot experiments,we conducted low temperature treatments for 7,14 and 21 days and studied the responses of the photosynthetic and antioxidant systems of different cold tolerant Bougainvillea to cold stress during these different periods.[Results](1)During the low-temperature treatment period,the initial fluorescence(F 0)of all varieties increased,with the most significant change in B.Paederia Foetida,which increased by 60.2%compared to day 0.Maximum fluorescence(F m),PSII effective photochemical electron production(F v/F m),and photosynthetic relative electron transfer rate(ETR)rapidly decreased and reached their lowest levels at day 21.As the intensification of low temperature stress,the photochemical quenching coefficient(qP)of the three Bougainvillea types decreased,while the non-photochemical quenching coefficient(qN)of B.Flaming Sumba and B.Paederia Foetida increased significantly.(2)After exposure to low temperature stress,the activities of SOD,POD and CAT enzymes in each variety decreased significantly and intensified with the extension of stress time,indicating that low temperature seriously inhibited the antioxidant enzyme activity of Bougainvillea.Among them,the SOD,POD and CAT enzyme activities of B.Paederia Foetida were higher than those of the other two varieties,indicating that B.Paederia Foetida had strong cold resistance.(3)In the AsA-GSH cycle system,the content of AsA and DHA of each Bougainvillea leaf significantly increased under low temperature.Compared to day 0,the GR activity of the three Bougainvillea types at day 21 increased by 180.91%,175.97%and 112.37%,respectively.Additionally,the AsA

关 键 词：抗氧化 低温 叶绿素荧光 AsA-GSH循环 三角梅 

分 类 号：Q945.78[生物学—植物学]