血清IP-10、STAT1表达水平在肾结石术后尿路感染中的临床诊断价值  

Clinical diagnostic value of serum IP-10 and STAT1 expression levels in urinary tract infection after nephrolithiasis surgery

作　　者：蒋多云 汪小明 李羽佳 蒋磊 伍春艳 吕高飞 文曙光 Jiang Duoyun;Wang Xiaoming;Li Yujia;Jiang Lei;Wu Chunyan;Lyu Gaofei;Wen Shuguang(Department of Urology,Yongzhou Central Hospital,Yongzhou 425000,China;Department of Urology,Second Affiliated Hospital of Guangxi Medical University,Nanning 530005,China;School of Nursing,Yongzhou Vocational and Technical College,Yongzhou 425000,China)

机构地区：[1]永州市中心医院泌尿外科,永州425000 [2]广西医科大学第二附属医院泌尿外科,南宁530005 [3]永州市职业技术学院护理学院,永州425000

出　　处：《国际泌尿系统杂志》2024年第1期93-97,共5页International Journal of Urology and Nephrology

摘　　要：目的探讨血清干扰素诱导蛋白-10(IP-10)、信号转导及转录活化因子1(STAT1)表达水平在肾结石患者术后尿路感染中的临床诊断价值。方法收集2019年3月至2021年12月在永州市中心医院和广西医科大学第二附属医院接受手术并住院的164例肾结石患者的临床资料。根据术后有无发生尿路感染将其分为未感染组(146例)和感染组(18例)。采用酶联免疫吸附(ELISA)法检测血清IP-10、STAT1水平,采用全自动微生物鉴定仪对尿液中的病原菌进行鉴定分析;采用受试者工作特征(ROC)曲线分析血清IP-10、STAT1表达水平对肾结石患者术后尿路感染的诊断价值;采用多因素logistic回归分析肾结石患者术后尿路感染的影响因素。结果18例尿路感染患者中共检出病原菌53株,其中革兰阳性菌18株,革兰阴性菌33株,真菌2株,尿路感染患者感染菌种以革兰阴性菌为主。感染组的住院时间、24 h尿量显著多于未感染组,血清IP-10、STAT1表达水平高于未感染组(均P<0.05)。血清IP-10、STAT1二者联合诊断肾结石患者术后尿路感染的受试者工作特征(ROC)曲线下面积(AUC)为0.893,特异度为89.0%,灵敏度为72.2%,均显著高于血清IP-10、STAT1单独诊断(均P<0.05)。多因素logistic回归分析结果显示,IP-10、STAT1是肾结石患者术后尿路感染的独立危险因素(均P<0.05)。结论血清IP-10、STAT1检测水平可作为肾结石患者术后尿路感染的诊断指标,二者联合可提高诊断效能,为临床制定干预措施提供参考。Objective To investigate the clinical value of serum interferon inducible protein-10(IP-10)and signal transducer and activator of transcription 1(STAT1)expression levels in the diagnosis of postoperative urinary tract infection in patients with nephrolithiasis.Methods Clinical data of 164 patients with kidney stones who underwent surgery and were hospitalized in Yongzhou Central Hospital and the Second Affiliated Hospital of Guangxi Medical University from March 2019 to December 2021 were collected.According to the occurrence of urinary tract infection,the patients were divided into uninfected group(146 cases)and infected group(18 cases).Enzyme-linked immunosorbent assay(ELISA)was performed to measure serum IP-10 and STAT1 levels,automatic microbial identification instrument was applied to identify and analyze pathogenic bacteria in urine;receiver operating characteristic(ROC)curve was performed to analyze the diagnostic value of serum IP-10 and STAT1 expression levels for postoperative urinary tract infection in patients with nephrolithiasis;multivariate logistic regression analysis was performed to analyze the influencing factors of postoperative urinary tract infection in patients with nephrolithiasis.Results A total of 53 pathogenic bacteria were detected in 18 patients with urinary tract infection,including 18 gram-positive bacteria,33 gram-negative bacteria,and 2 fungi.In this study,patients with urinary tract infection were mainly infected with Gram-negative bacteria.The length of hospital stay and 24 h urine volume in the infected group were significantly higher than those in the uninfected group,and the expression levels of serum IP-10 and STAT1 were higher than those in the uninfected group(all P<0.05).The areas under the curve(AUC)of serum IP-10,STAT1 and their combination in the diagnosis of postoperative urinary tract infection in patients with nephrolithiasis were 0.844,0.785 and 0.893,respectively,the specificities were 81.5%,65.8%and 89.0%,respectively,and the sensitivities were 77.8%,88.9%and 72.

关 键 词：肾结石 干扰素诱导蛋白-10 信号转导转录活化因子1 尿路感染 

分 类 号：R699.2[医药卫生—泌尿科学]