Conductive proteins-based extracellular electron transfer of electroactive microorganisms  

作　　者：Junqi Zhang Zixuan You Dingyuan Liu Rui Tang Chao Zhao Yingxiu Cao Feng Li Hao Song 

机构地区：[1]Frontier Science Center for Synthetic Biology(Ministry of Education),Key Laboratory of Systems Bioengineering,and School of Chemical Engineering and Technology,Tianjin University,Tianjin,China

出　　处：《Quantitative Biology》2023年第4期405-420,共16页定量生物学（英文版）

基　　金：National Key Research and Development Program of China,Grant/Award Number:2018YFA0901300;National Natural Science Foundation of China,Grant/Award Numbers:22378305,32071411,32001034,21621004;Tianjin Science and Technology Plan Project,Grant/Award Number:20JCQNJC00830。

摘　　要：Electroactive microorganisms(EAMs)could utilize extracellular electron transfer(EET)pathways to exchange electrons and energy with their external surroundings.Conductive cytochrome proteins and nanowires play crucial roles in controlling electron transfer rate from cytosol to extracellular electrode.Many previous studies elucidated how the c-type cytochrome proteins and conductive nanowires are synthesized,assembled,and engineered to manipulate the EET rate,and quantified the kinetic processes of electron generation and EET.Here,we firstly overview the electron transfer pathways of EAMs and quantify the kinetic parameters that dictating intracellular electron production and EET.Secondly,we systematically review the structure,conductivity mechanisms,and engineering strategies to manipulate conductive cytochromes and nanowire in EAMs.Lastly,we outlook potential directions for future research in cytochromes and conductive nanowires for enhanced electron transfer.This article reviews the quantitative kinetics of intracellular electron production and EET,and the contribution of engineered c-type cytochromes and conductive nanowire in enhancing the EET rate,which lay the foundation for enhancing electron transfer capacity of EAMs.

关 键 词：c-type cytochromes conductive nanowires extracellular electron transfer kinetic parameters synthetic biology 

分 类 号：Q2[生物学—细胞生物学]