脾气虚型免疫性血小板减少症小鼠模型与白细胞介素-27、白细胞介素-10的表达  

Mouse Model of Iimmune Thrombocytopenia with Spleen Qi Deficiency and Expressions of Interleukin-27 and Interleukin-10

作　　者：赵倩 严香 张雅月[2] ZHAO Qian;YAN Xiang;ZHANG Yayue(Beijing University of Chinese Medicine,Beijing 100029,China;Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China)

机构地区：[1]北京中医药大学,北京100029 [2]北京中医药大学东直门医院,北京100700

出　　处：《世界中医药》2023年第24期3508-3511,3517,共5页World Chinese Medicine

基　　金：国家自然科学基金青年科学基金项目(81703903);北京中医药大学东直门医院青苗人才培养计划(DZMYS-201807);北京中医药大学东直门医院科技创新专项(DZMKJCX-2022-007)。

摘　　要：目的:研究脾气虚型免疫性血小板减少症(ITP)模型小鼠血清与脾脏组织中白细胞介素-27(IL-27)、白细胞介素-10(IL-10)的表达水平及意义。方法:24只白变种实验小鼠以区间分组法随机分为对照组、模型组、泼尼松组、健脾益气摄血组。除对照组外,其余组均以被动免疫造模法建立脾气虚型ITP小鼠模型。造模后,模型组、对照组用生理盐水灌胃,泼尼松组、健脾益气摄血组分别用泼尼松、健脾益气摄血颗粒灌胃。各组均灌胃1次/d,连续8 d。采用酶联免疫吸附试验检测外周血中血小板计数及血清与脾脏中IL-27、IL-10表达水平。结果:与对照组比较,模型组血清和脾脏IL-27、IL-10均显著降低(均P<0.01)。与模型组比较,泼尼松组、健脾益气摄血组血清和脾脏IL-10均明显升高(P<0.05);泼尼松组、健脾益气摄血组血清与脾脏IL-27均有所升高,但差异均无统计学意义(均P>0.05)。结论:IL-27、IL-10可能在脾气虚型ITP中发挥保护性作用,且发挥作用的主要场所在外周血而非脾脏;此外泼尼松与健脾益气摄血颗粒治疗ITP的机制可能与提高IL-10的含量有关,而与提高IL-27的含量关系不大。Objective:To study the expressions of interleukin-27(IL-27)and interleukin-10(IL-10)in serum and spleen tissue of immune thrombocytopenia(ITP)model mice with spleen deficiency and their significance.Methods:A total of 24 BALB/c mice were divided into control group,model group,prednisone group,and Jianpi Yiqi Shexue group by interval grouping method.Except for the control group,the other three groups received passive immunity to establish ITP model with spleen qi deficiency.After modeling,the model group and the control group were given normal saline by gavage,while the two treatment groups were given prednisone and Jianpi Yiqi Shexue Granules by gavage.Each group was orally administered once a day for 8 consecutive days.The platelet count in peripheral blood and the expressions of IL-27 and IL-10 in serum and spleen were detected by enzyme linked immunosorbent assay.Results:Compared with the conditions in the control group,IL-27 and IL-10 in serum and spleen of the model group were decreased(P<0.01).Compared with the model group,both treatment groups had elevated IL-10 in serum and spleen(P<0.05),and up-regulated IL-27(P>0.05).Conclusion:IL-27 and IL-10 may play a protective role in ITP with spleen qi deficiency,mainly acting in peripheral blood rather than the spleen.In addition,the mechanism of prednisone and Jianpi Yiqi Shexue Granules in treating ITP may be related to increasing IL-10,but not to increasing IL-27.

关 键 词：脾气虚 原发性免疫性血小板减少症 小鼠模型 白细胞介素-10 白细胞介素-27 血清 脾脏组织 健脾益气摄血颗粒 

分 类 号：R259[医药卫生—中西医结合]