夏桑菊颗粒体外抗人冠状病毒研究  

Inhibitory of Xiasangju Granules on Human Coronavirus

作　　者：孙维广 崔晓兰[2] 黄志云 贡磊磊 时宇静[2] 高建胜 袁孟菲 毛鑫 SUN Weiguang;CUI Xiaolan;HUANG Zhiyun;GONG Leilei;SHI Yujing;GAO Jiansheng;YUAN Mengfei;MAO Xin(Guangzhou Baiyunshan Xingqun Pharmaceutical Co.,Ltd.,Guangzhou 510288,China;Institute of Chinese Meteria Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Beijing Obstetrics and Gynecology Hospital,Capital Medical University,Beijing 100026,China)

机构地区：[1]广州白云山星群〔药业〕股份有限公司,广州510288 [2]中国中医科学院中药研究所,北京100700 [3]首都医科大学附属北京妇产医院药学部,北京100026

出　　处：《世界中医药》2023年第24期3518-3522,共5页World Chinese Medicine

基　　金：国家自然科学基金青年科学基金项目(82204698);中国中医科学院科技创新工程项目(CI2021B015)。

摘　　要：目的:研究夏桑菊颗粒对人冠状病毒-229E(HCoV-229E)的抑制作用。方法:采用细胞病变(CPE)法检测夏桑菊颗粒对A549细胞的安全剂量,采用CPE法检测夏桑菊颗粒对HCoV-229E引起A549细胞病变的影响,采用反转录聚合酶链式反应(RT-PCR)法检测夏桑菊颗粒对HCoV-229E于A549细胞中的复制作用;采用噻唑蓝(MTT)法检测夏桑菊颗粒对Huh-7细胞的安全剂量;采用RT-PCR法检测夏桑菊颗粒对HCoV-229E感染引起Huh-7细胞炎症介质表达的影响。结果:夏桑菊颗粒对于HCoV-229E感染A549细胞引起CPE的半数抑制浓度为23.78 mg生药/mL,选择指数为2.0,夏桑菊颗粒能够剂量依赖性地抑制A549细胞内HCoV-229E病毒载量,夏桑菊颗粒可以抑制HCoV-229E诱导Huh7细胞内炎症介质单核细胞趋化蛋白-1(MCP-1)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和白细胞介素-8(IL-8)的mRNA过度表达。结论:夏桑菊颗粒具有明显的抗HCoV-229E体外感染作用。Objective:To study the inhibitory effect of Xiasangju Granules on human coronavirus-229E(HCoV-229E).Methods:The safe dose of Xiasangju Granules on A549 cells was detected by the cytopathic effect(CPE)method,and the effect of Xiasangju Granules on cytopathic effect of A549 cells caused by HCoV-229E was detected by the CPE method.Reverse transcription polymerase chain reaction(RT-PCR)method was used to detect the effects of Xiasangju Granules on the replication of HCoV-229E in A549 cells.The safe dose of Xiasangju Granules on Huh-7 cells was detected by Thiazolyl Blue(MTT)method,and RT-PCR method was used to detect the effects of Xiasangju Granules on the expression of inflammatory mediators in Huh-7 cells caused by HCoV-229E.Results:The median inhibitory concentration of Xiasangju Granules on CPE of A549 cells infected by HCoV-229E is 23.78 mg crude drug/mL,and the selection index is 2.0.Xiasangju Granules can dose-dependently inhibit the viral load of HCoV-229E in A549 cells and inhibit the mRNA overexpression of monocyte chemoattractant protein-1(MCP-1),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),and interleukin-8(IL-8)induced by HCoV-229E in Huh-7 cells Conclusion:Xiasangju Granules have obvious anti-infection effects of HCoV-229E in vitro.

关 键 词：夏桑菊颗粒 抗病毒 冠状病毒 细胞因子 A549细胞 HUH-7细胞 

分 类 号：R285.5[医药卫生—中药学]