参元丹胶囊对气虚血瘀型斑马鱼的治疗作用  

Effect of Shenyuandan Capsules on Zebrafish Model of Qi Deficiency and Blood Stasis

作　　者：解长旭 郭帅杰 陈思琪 李思耐[1,2] 张蕾 刘卫红[1,2] 刘红旭 周明学[1,2] XIE Changxu;GUO Shuaijie;CHEN Siqi;LI Sinai;ZHANG Lei;LIU Weihong;LIU Hongxu;ZHOU Mingxue(Beijing Hospital of Traditional Chinese Medicine,Capital Medical University,Beijing 100010,China;Beijing Institute of Traditional Chinese Medicine,Beijing 100010,China)

机构地区：[1]首都医科大学附属北京中医医院,北京100010 [2]北京市中医药研究所,北京100010

出　　处：《世界中医药》2023年第24期3532-3537,共6页World Chinese Medicine

基　　金：国家自然科学基金项目(82274287);北京市自然科学基金项目(7232266)。

摘　　要：目的:探讨参元丹胶囊对气虚血瘀型斑马鱼的干预作用。方法:采用无水亚硫酸钠和异丙肾上腺素盐酸盐溶液处理受精后第4天野生型AB品系斑马鱼,建立气虚血瘀斑马鱼模型,评价参元丹胶囊对其的治疗作用。实验设正常对照组、模型对照组、脑心通胶囊对照组、参元丹胶囊低、中、高剂量组。通过测定斑马鱼总运动距离、斑马鱼体内腺苷三磷酸(ATP)含量、乳酸含量、心脏血流速度、心输出量以及对心肌细胞凋亡程度,评价参元丹胶囊对气虚血瘀型斑马鱼的改善作用。结果:与正常对照组比较,模型组斑马鱼总运动距离、ATP含量、血流速度、心输出量均显著降低,体内乳酸含量和心肌细胞凋亡程度均显著提高(均P<0.05),与模型组比较,参元丹胶囊组斑马鱼运动距离、体内ATP含量、心输出量及血流速度均显著增加,体内乳酸含量、心肌细胞凋亡程度均显著降低(均P<0.05)。结论:参元丹胶囊对于气虚血瘀模型斑马鱼具有较好的治疗作用。Objective:To investigate the therapeutic effect of Shenyuandan Capsules on the zebrafish model of qi deficiency and blood stasis.Methods:Anhydrous sodium sulfite and isoprenaline hydrochloride solution were used to treat the wild-type AB strain of zebrafish on the 4th day after fertilization to establish a model of qi deficiency and blood stasis.The therapeutic effect of Senyuandan Capsules on the model was evaluated.The experiments were carried out for the following groups:normal control,model control,Naoxintong Capsules,and low-,medium-,and high-dose Shenyuandan Capsules.The effect of Shenyuandan Capsules on the model was evaluated based on the total moving distance,levels of adenosine triphosphate(ATP)and lactic acid,cardiac blood flow velocity,cardiac output,and apoptosis of cardiomyocytes in the zebrafish.Results:Compared with the normal control group,the model control group showed reductions in the total moving distance,ATP level,blood flow velocity,and cardiac output and increases in the lactic acid level and cardiomyocyte apoptosis(all P<0.05).Compared with the model control group,Shenyuandan Capsules restored the indicators above(all P<0.05).Conclusion:Shenyuandan Capsules can significantly attenuate the syndrome of qi deficiency and blood stasis in zebrafish.

关 键 词：气虚血瘀 参元丹胶囊 斑马鱼 冠心病 心肌缺血 证候模型 无水亚硫酸钠 异丙肾上腺素盐酸盐 

分 类 号：R285.5[医药卫生—中药学]