饲料酵母水溶物具有促进草鱼原代肝细胞生长及修复过氧化氢损伤的作用  

Effect of Yeast-Derived Feed Additive Aqueous Solution on Promoting Growth and Repairing Hydrogen Peroxide Damage in Grass Carp Primary Hepatocytes

作　　者：马杰 王卓君 石瑶瑶 叶元土[1,2] 蔡春芳 吴萍[1,2] 陈鹏 Ma Jie;Wang Zhuojun;Shi Yaoyao;Ye Yuantu;Cai Chunfang;Wu Ping;Chen Peng(School of Biology&Basic Medical Sciences,Soochow University ,Suzhou 215123;Key Laboratory of Aquatic Animal Nutrition ,Suzhou 215123;Beijing Enhalor Institute of Biotechnology ,Beijing 100081)

机构地区：[1]苏州大学基础医学与生物科学学院,苏州215123 [2]江苏省水产动物营养重点实验室,苏州215123 [3]北京英惠尔生物技术有限公司,北京100081

出　　处：《中国粮油学报》2024年第1期159-166,共8页Journal of the Chinese Cereals and Oils Association

摘　　要：研究以3种酵母水溶物为实验材料,探究饲料酵母对草鱼原代肝细胞生长的促进作用和过氧化氢(H_(2)O_(2))诱导草鱼原代肝细胞氧化损伤的缓解作用。实验使用酶消化法分离得到草鱼原代肝细胞,以200μmol/L H_(2)O_(2)处理细胞1 h建立氧化应激模型;分别选取质量浓度为25、50、100、200、400 mg/L的饲料酵母水溶物处理细胞,比较3种饲料酵母水溶物对原代肝细胞的促生长效果;分别使用3种饲料酵母的最佳促生长质量浓度处理模型细胞,分析饲料酵母水溶物对H_(2)O_(2)诱导的氧化损伤的缓解作用。结果表明:3种饲料酵母水溶物均能促进原代肝细胞增殖,酵母培养物YC-A、YC-B、酵母细胞壁多糖YCP的最佳促生长质量浓度分别为100、200、100 mg/L,在该质量浓度下细胞活力较对照组极显著提高(P<0.001)。相比于H_(2)O_(2)模型组,3个饲料酵母水溶物组细胞活力和MMP极显著提高(P<0.001),培养液中LDH活性极显著降低(P<0.001),细胞凋亡率和胞内ROS水平极显著降低(P<0.01);电镜结果显示,模型组细胞线粒体肿胀、嵴扭曲或消失,细胞内出现大量空泡,而3个饲料酵母水溶物处理组观察到线粒体肿胀情况得以改善。转录组结果提示,YCP可能通过增加细胞内Ⅰ相和Ⅱ相代谢酶保护细胞免受氧化损伤,而YC对细胞的保护可能是通过调控细胞内铁稳态实现的。饲料酵母水溶物能够促进草鱼原代肝细胞生长,并对原代肝细胞H_(2)O_(2)氧化损伤具有一定的修复作用。In this experiment,three kinds of yeast-derived feed additive aqueous solutions were used as the test materials,aimming to investigate the promoting effect of yeast-derived feed additives on the growth of grass carp primary hepatocytes and the alleviating effect of hydrogen peroxide(H_(2)O_(2))on the oxidative damage of grass carp primary hepatocytes.Grass carp primary hepatocytes were obtained by the enzymatic digestion method and plated at 200μmol/L H_(2)O_(2) for 1 h to establish an oxidative stress model.The cells were treated with yeast-derived feed additive aqueous solution at concentrations of 25,50,100,200,and 400 mg/L,to compare the growth promoting effects of three yeast-derived feed additive aqueous solutions on primary hepatocytes.The model cells were treated using the optimal growth promoting concentration of three yeast-derived feed additives,respectively,and the alleviating effect of yeast-derived feed additives on H_(2)O_(2) induced oxidative damage was analyzed.The results indicated that three yeast-derived feed additive aqueous solutions could promote the proliferation of primary hepatocytes,and the optimal growth promoting concentrations of yeast culture YC-A,YC-B and yeast cell wall polysaccharide YCP were 100,200 and 100 mg/L,respectively,at which cell viability was significantly increased compared with the control group(P<0.001).Compared to the H_(2)O_(2) group,the three yeast-derived feed additive groups exhibited highly significant improvements in cell viability and MMP(P<0.001),highly significant reductions in LDH activity in the culture medium(P<0.001),and highly significant reductions in the cell apoptosis rate and intracellular ROS levels(P<0.01).Transmission electron microscopy revealed swollen,distorted,or absent mitochondria and numerous vacuoles in the cells of the H_(2)O_(2) group,whereas improved mitochondrial swelling was observed in the three yeast-derived feed additive aqueous solution-treated groups.The transcriptome results suggested that YCP may protect cells from oxidativ

关 键 词：饲料酵母 原代肝细胞 过氧化氢 氧化损伤 草鱼 

分 类 号：S963[农业科学—水产养殖]