利用分子标记技术鉴定甜菜单胚不育系的混杂程度  

Identification of the Degree of Confounding in Sugar Beet Monogerm Sterile Lines Using Molecular Markers

作　　者：陈姝源 吴则东[1] CHEN Shu-yuan;WU Ze-dong(School of Modern Agriculture and Eco-Environment,Heilongjiang University,Harbin 150080)

机构地区：[1]黑龙江大学现代农业与生态环境学院,哈尔滨150080

出　　处：《中国甜菜糖业》2023年第4期1-4,共4页China Beet & Sugar

基　　金：财政部和农业农村部国家现代农业产业技术体系(糖料)项目(CARS-170111)资助;黑龙江大学创新创业训练计划项目共同资助。

摘　　要：在甜菜单胚雄性不育系和保持系收获的过程中,由于人为的原因会造成不育系中混有保持系以及保持系中混有不育系的现象,而不育系中混有保持系会对二元不育系的纯度以及杂交种的杂交程度都会造成重大影响,因此本研究利用分子标记技术对来自新疆以及黑龙江的24份不育系中是否混有保持系进行鉴定,了解我国目前不育系的纯度。试验结果表明,在这24份不育系中,有22份经常使用的甜菜单胚不育系的不育程度达到100%,可以直接用于甜菜育种中;另外两份由黑龙江大学新改良的甜菜单胚不育系出现了混杂的现象,后续应将混杂株进行剔除后再进行纯度鉴定,待育性达到要求后再进行配合力的测定。In beet single embryo male sterile line and keep in the process of harvest,due to artificial causes in the sterile line and keep the phenomenon of sterile line,and keep the purity of binary sterile line and hybrid hybrid degree will cause significant impact,so this study intends to use molecular marker technology from Xinjiang and Heilongjiang 24 sterile lines mixed with the identification,understand the purity of sterile lines in China.The test results show that in 22 of the sterile lines is 100% and can be used in beet breeding;in the other two,the purity identification and the coordination force should be determined after the fertility reaches the requirement.

关 键 词：甜菜 TR1 分子标记 育性鉴定 基因型 

分 类 号：S566.3[农业科学—作物学]