对饮用水中疑似铜绿假单胞菌的复核鉴定  

Research on the Re Identification of Suspected Pseudomonas aeruginosa in Drinking Water

作　　者：田雨 周鹏飞 吴海平 TIAN Yu;ZHOU Pengfei;WU Haiping(Sinolight Inspection&Certification CO.,Ltd,Beijing 100016,China)

机构地区：[1]中轻检验认证有限公司,北京100016

出　　处：《工业微生物》2024年第1期177-183,共7页Industrial Microbiology

摘　　要：铜绿假单胞菌是一种不允许在饮用水中检出的条件致病菌,本文对10株分离自饮用水样品的菌株进行了菌落形态观察及生化反应确证,并使用HK-MID细菌鉴定系统、VITEK 2全自动微生物鉴定系统和16S rRNA基因测序等多种手段进行进一步鉴定。鉴定结果表明,部分菌株并非铜绿假单胞菌,但均为条件致病菌,从食品安全的角度考虑,不应出现在饮用水、矿泉水中。本文除了探讨以往的研究普遍关注的检验方法的准确性与时效性,还对这些菌株进行了分析,为相关管理部门进行质量监督、制定标准提供了研究依据和借鉴。Pseudomonas aeruginosa is a conditional pathogenic bacterium that is not allowed to be detected in drinking water.This paper observed the colony morphology and confirmed biochemical reactions of 10 single colonies isolated from drinking water samples.Further identification was carried out using various methods such as HK-MID bacterial biochemical identification system,VITEK 2 fully automated microbial identification system,and 16S rRNA gene sequencing.The identification results indicate that some strains are not Pseudomonas aeruginosa,but rather opportunistic pathogens.From the perspective of food safety,these should not appear in drinking water or mineral water.In addition to exploring the accuracy and timeliness of testing methods that have been widely studied in previous studies,this paper also analyzes these strains,providing research basis and reference value for quality supervision and standard formulation by relevant management departments.

关 键 词：铜绿假单胞菌 HK-MID细菌生化鉴定系统 VITEK 2全自动微生物鉴定系统 16S rRNA基因测序 

分 类 号：R123.1[医药卫生—环境卫生学]