4种马尾藻附生菌群结构比较研究  

COMPARISON OF EPIPHYTIC MICROBIAL COMMUNITIES ON FOURSPECIES OF SARGASSUM

作　　者：郭战胜[1,2] 陈雯静 常丽荣 梁振林 施坤涛[3] GUO Zhan-Sheng;CHEN Wen-Jing;CHANG Li-Rong;LIANG Zhen-Lin;SHI Kun-Tao(Marine College,Shandong University,Weihai 264200,China;Weihai Changqing Ocean Science Technology Co.,LTD.,Rongcheng 264300,China;Huancui District Marine Development Research Center,Weihai 264200,China)

机构地区：[1]山东大学海洋学院,威海264200 [2]威海长青海洋科技股份有限公司,荣成264300 [3]威海市环翠区海洋发展研究中心,威海264200

出　　处：《水生生物学报》2024年第4期643-652,共10页Acta Hydrobiologica Sinica

基　　金：国家重点研发计划(2016YFE0205700);山东省现代农业产业技术体系刺参产业技术体系(SDAIT-22-14)资助。

摘　　要：海黍子(Sargassum muticum,SM)、鼠尾藻(Sargassum thunbergii,ST)、铜藻(Sargassum horneri,SH)和海蒿子(Sargassum pallidum,SP)是我国黄渤海常见的大型马尾藻属海藻,研究以靖子湾潮间带4种马尾藻为研究对象,自然海水(W)作为对照,利用16S rRNA基因扩增子高通量测序技术比较4种马尾藻类附生菌群的结构。研究结果表明,ST附生菌群多样性和物种丰富度最高,SH最低。NMDS(无度量多维标定法)和PERMANOVA分析表明4种马尾藻附生菌的群落结构与海水中微生物群落结构存在显著性差异(P<005),而不同马尾藻之间差异却不显著。LEfse分析共识别出18个Biomarkers,其中W、SH、SM和ST样本分别有9、4、3和2个。变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidota)为4种马尾藻附生菌群的主要优势菌门,相对丰度为80.39%—94.54%;基于属水平和相对丰度前10ASVs的群落结构组成特征表明,4种马尾藻附生菌群结构组成存在明显差异,呈现出宿主特异性。利用Tax4Fun软件对4种马尾藻类附生菌群生态功能进行预测,在一级功能水平上,新陈代谢(Metabolism)、遗传信息处理(Genetic Information Processing)为主要功能;在二级功能水平上,共注释到44个二级代谢通路,其中碳水化合物代谢(Carbohydrate metabolism)相对丰度最高。研究通过比较4种马尾藻附生菌群结构多样性,为进一步深入了解马尾藻属的生态作用提供理论依据。The macroalgae,including Sargassum muticum(labeled as SM),Sargassum thunbergii(ST),Sargassum horneri(SH)and Sargassum pallidum(SP),are common Sargassum species distributed in the intertidal zone of Yellow Sea and Bohai Sea in China.These Sargassum species serve as essential components of seaweed beds,creating an optimal habitat for marine life,playing an important role in the conservation of fishery resources,marine eutrophication,and improvement of the ecological environment in coastal waters.The close interactions between macroalgae and their epiphytic microorganisms have a significant impact on the growth and development of the host macroalgae.Consequently,describing the microbial diversity associated with Sargassum species is an essential step towards a comprehensive understanding of gulfweed ecosystem dynamics.Previous studies primarily focused on individual Sargassum species,leaving a notable gap in the comparison of microbial diversity among closely related sympatric host species.In this study,the epiphytic microbial communities on four sympatric Sargassum species(SM,ST,SH,and SP)and the overlaying seawater(labeled as W)were investigated by high-throughput sequencing of 16S rRNA for the first time.The results showed that a total of 8793 ASVs were obtained from 15 samples,only 12 ASVs were shared between macroalgae and water sample groups.ST displayed the highest number unique ASVs among Sargassum groups.The alpha diversity analyses showed that the microbial richness and diversity were obviously higher in water than that in macroalgae.Comparing the Sargassum groups,ST displayed the highest Shannon and Chao 1 values.The NMDS analysis showed that replicates exhibited high similarity,except for the SH group,as the microbial communities in macroalgae and water groups formed clear separate clusters.Further PERMANOVA analysis confirmed no significant differences between Sargassum groups(P>0.05).The composition of microbial communities on macroalgae were assessed at both the phylum and genus levels.Dominant phyla

关 键 词：附生菌群 高通量测序 多样性 功能预测 马尾藻 

分 类 号：Q938.8[生物学—微生物学]