Promotion of reactive oxygen species activated by nanosilver surface engineering for resistant bacteria-infected skin tissue therapy  

作　　者：Pei-Pei Li Yu Zhang Chao Wang Shu-Jie Wang Wen-Qing Yan Dou-Xin Xiao Jing Kang De-Zhi Yang Hai-Xia Wu Alideertu Dong 

机构地区：[1]College of Chemistry and Chemical Engineering,Inner Mongolia University,Hohhot 010021,China [2]Engineering Research Center of Dairy Quality and Safety Control Technology,Ministry of Education,Inner Mongolia University,Hohhot 010021,China [3]International Mongolia Hospital of Inner Mongolia,Hohhot 010065,China

出　　处：《Rare Metals》2023年第12期4167-4183,共17页稀有金属（英文版）

基　　金：financially supported by the National Natural Science Foundation of China (Nos.22062017 and 22164015);the Inner Mongolia Autonomous Region Program for Key Science and Technology (No.2020GG0161);the Natural Science Foundation of Inner Mongolia Autonomous Region (No.2019JQ03);the Ordos City Program for Key Science and Technology (No.2022YY003);the Open Project of State Key Laboratory of Supramolecular Structure and Materials (No.sklssm2022021);the Program of Higher-Level Talents of Inner Mongolia University (No.10000-22311201/035);the Science and Technology Research Projects in Colleges and Universities of Inner Mongolia Autonomous Region (No.NJZZ23091)。

摘　　要：Nanosilver has been regarded as a promising alternative to traditional antibiotics for fighting pathogenassociated infections due to its efficacy toward a broad spectrum of pathogens.However,bacterial resistance to nanosilver has emerged recently.In this contribution,a surface engineering strategy based on N-halamine chemistry to address bacterial resistance to nanosilver was proposed.Using 1,3-dichloro-5,5-dimethylhydantoin(DCDMH)as an N-halamine source,AgCI nanodots were deposited on the surface of Ag nano wires(Ag NWs)via in situ redox reaction to prepare AgCl-on-Ag NWs.After in vitro and in vivo tests,AgCl-on-Ag NWs effectively inactivated two antibiotic-resistant bacteria,ampicillinresistant Escherichia coli(AREC)and methicillin-resistant Staphylococcus aureus(MRSA)with the minimum bactericidal concentration(MBC)as low as 10μg·ml~(-1)and exhibited good biosafety against normal cells.The experimental and theoretical tests demonstrated that AgCl-onAg NWs worked on AREC and MAS A by generating high level of reactive oxygen species under visible light irradiation,coupled with the sustained Ag ion release.Meanwhile,the antibacterial mechanism of AgCl-on-Ag NWs against MRSA was verified at the gene level by transcriptome analysis(RNA sequencing).Moreover,the fullthickness defect model verified that AgCl-on-Ag NWs reduced inflammatory cell infiltration and dramatically accelerated wound healing.This work provides a synergistic mechanism based on nanosilver surface engineering to eradicate the resistant bacteria that can alleviate drug resistance and develop an innovative approach for the treatment of bacterial infections.

关 键 词：Surface engineering NANOSILVER N-HALAMINE Reactive oxygen species(ROS) Antibiotic resistance 

分 类 号：R446.5[医药卫生—诊断学] TB383.1[医药卫生—临床医学]