Functional evidence that FGFR regulates MAPK signaling in organizer specification in the gastropod mollusk Lottia peitaihoensis  

作　　者：Sujian Tan Pin Huan Baozhong Liu 

机构地区：[1]CAS and Shandong Province Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China [2]Laboratory for Marine Biology and Biotechnology,Laoshan Laboratory,Qingdao 266237,China

出　　处：《Marine Life Science & Technology》2023年第4期455-466,共12页海洋生命科学与技术（英文）

基　　金：supported by the National Natural Science Foundation of China(grant numbers 42206092,42076123);the Earmarked Fund for China Agriculture Research System(grant number CARS-49);the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)(No.LSKJ202203002).

摘　　要：The D-quadrant organizer sets up the dorsal–ventral(DV)axis and regulates mesodermal development of spiralians.Studies have revealed an important role of mitogen-activated protein kinase(MAPK)signaling in organizer function,but the related molecules have not been fully revealed.The association between fibroblast growth factor receptor(FGFR)and MAPK signaling in regulating organizer specification has been established in the annelid Owenia fusiformis.Now,comparable studies in other spiralian phyla are required to decipher whether this organizer-inducing function of FGFR is prevalent in Spiralia.Here,we indicate that treatment with the FGFR inhibitor SU5402 resulted in deficiency of organizer specification in the mollusk Lottia peitaihoensis.Subsequently,the bone morphogenetic protein(BMP)signaling gradient and DV patterning were disrupted,suggesting the roles of FGFR in regulating organizer function.Changes in multiple aspects of organizer function(the morphology of vegetal blastomeres,BMP signaling gradient,expression of DV patterning markers,etc.)indicate that these developmental functions have different sensitivities to FGFR/MAPK signaling.Our results reveal a functional role of FGFR in organizer specification as well as DV patterning of Lottia embryos,which expands our knowledge of spiralian organizers.

关 键 词：FGF signaling Organizer specification BMP signaling gradient DV patterning MOLLUSK 

分 类 号：Q954[生物学—动物学]