Transcriptional regulation analysis reveals the complexity of metamorphosis in the Pacific oyster(Crassostrea gigas)  

作　　者：Fei Xu Shaoxi Deng Daria Gavriouchkina Guofan Zhang 

机构地区：[1]CAS and Shandong Province Key Laboratory of Experimental Marine Biology,Center for Ocean Mega-Science,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China [2]Laoshan Laboratory,Qingdao 266237,China [3]Shandong Technology Innovation Center of Oyster Seed Industry,Qingdao 266105,China [4]School of Energy and Environment and State Key Laboratory of Marine Pollution,City University of Hong Kong,Hong Kong 999077,China [5]Molecular Genetics Unit,Okinawa Institute of Science and Technology Graduate University,Okinawa 904-0495,Japan [6]UK Dementia Research Institute,University College London,London WC1E 6BT,UK

出　　处：《Marine Life Science & Technology》2023年第4期467-477,共11页海洋生命科学与技术（英文）

基　　金：supported by the Oceanographic Data Center,IOCAS.We acknowledge financial support from the Science&Technology Innovation Project of Laoshan Laboratory(LSKJ202203001);the Key Research and Development Program of Shandong(2022LZGC015),and the Taishan Scholars Program.

摘　　要：Many marine invertebrate phyla are characterized by indirect development.These animals transit from planktonic larvae to benthic spats via settlement and metamorphosis,which contributes to their adaption to the marine environment.Studying the biological process of metamorphosis is,thus,key to understanding the origin and evolution of indirect development.Although numerous studies have been conducted on the relationship between metamorphosis and the marine environment,microorganisms,and neurohormones,little is known about gene regulation network(GRN)dynamics during metamorphosis.Metamorphosis-competent pediveligers of the Pacific oyster Crassostrea gigas were assayed in this study.By assaying gene expression patterns and open chromatin region changes of different samples of larvae and spats,the dynamics of molecular regulation during metamorphosis were examined.The results indicated significantly different gene regulation networks before,during and post-metamorphosis.Genes encoding membrane-integrated receptors and those related to the remodeling of the nervous system were upregulated before the initiation of metamorphosis.Massive biogenesis,e.g.,of various enzymes and structural proteins,occurred during metamorphosis as inferred from the comprehensive upregulation of the protein synthesis system post epinephrine stimulation.Hierarchical downstream gene networks were then stimulated.Some transcription factors,including homeobox,basic helix–loop–helix and nuclear receptors,showed different temporal response patterns,suggesting a complex GRN during the transition stage.Nuclear receptors,as well as their retinoid X receptor partner,may participate in the GRN controlling oyster metamorphosis,indicating an ancient role of the nuclear receptor regulation system in animal metamorphosis.

关 键 词：Gene regulation network Indirect development LOPHOTROCHOZOA Nuclear receptors NEUROHORMONES Protein synthesis Transcription factors 

分 类 号：Q178.53[生物学—水生生物学] Q95[生物学—普通生物学]