Evolutionary traits and functional roles of chemokines and their receptors in the male pregnancy of the Syngnathidae  

作　　者：Han Jiang Zhanwei Zhao Haiyan Yu Qiang Lin Yali Liu 

机构地区：[1]CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China [2]Guangdong Provincial Key Laboratory of Applied Marine Biology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China [3]University of Chinese Academy of Sciences,Beijing 101400,China

出　　处：《Marine Life Science & Technology》2023年第4期500-510,共11页海洋生命科学与技术（英文）

基　　金：supported by the National Natural Science Foundation of China(41825013);the Marine Economic Development Project(GDNRC[2022]36);National Key R&D Program of China(2022YFC3102403);National Natural Science Foundation of China(42230409,42276127);Guangdong Basic and Applied Basic Research Foundation(2021A1515011380,2021A1515011393).

摘　　要：Vertebrates have developed various modes of reproduction,some of which are found in Teleosts.Over 300 species of the Syngnathidae(seahorses,pipefishes and seadragons)exhibit male pregnancies;the males have specialized brood pouches that provide immune protection,nourishment,and oxygen regulation.Chemokines play a vital role at the mammalian maternal–fetal interface;however,their functions in fish reproduction are unclear.This study revealed the evolutionary traits and potential functions of chemokine genes in 22 oviparous,ovoviviparous,and viviparous fish species through comparative genomic analyses.Our results showed that chemokine gene copy numbers and evolutionary rates vary among species with different modes of reproduction.Syngnathidae lost cxcl13 and cxcr5,which are involved in key receptor–ligand pairs for lymphoid organ development.Notably,Syngnathidae have site-specific mutations in cxcl12b and ccl44,suggesting immune function during gestation.Moreover,transcriptome analysis revealed that chemokine gene expression varies among Syngnathidae species with different types of brood pouches,suggesting adaptive variations in chemokine functions among seahorses and their relatives.Furthermore,challenge experiments on seahorse brood pouches revealed a joint immune function of chemokine genes during male pregnancy.This study provides insights into the evolutionary diversity of chemokine genes associated with different reproductive modes in fish.

关 键 词：Reproductive mode Immune response SEAHORSE Brood pouch 

分 类 号：Q492.6[生物学—生理学]