基于色泽与多指标成分分析的川明参质量快速评价研究  

Study on Rapid Quality Assessment of Chuanminshen violaceum Sheh et Shan Based on Color and Multi-Index Component Determination

作　　者：向缅 胡平[1] 张美[1] 林娟 周霞 杨玉霞[1] 周先建[1] XIANG Mian;HU Ping;ZHANG Mei;LIN Juan;ZHOU Xia;YANG Yuxia;ZHOU Xianjian(Sichuan Academy of Chinese Medicine Sciences,Chengdu,Sichuan 610041,China)

机构地区：[1]四川省中医药科学院,四川成都610041

出　　处：《西昌学院学报（自然科学版）》2023年第4期6-12,共7页Journal of Xichang University(Natural Science Edition)

基　　金：四川省科技厅重点研发项目(2022YFS0592)。

摘　　要：[目的]探索川明参外观色泽与多个指标成分间的关系,为快速、高效筛选优质川明参药材提供参考。[方法]以不同商品规格川明参药材为研究对象,首先利用分光测色仪测定川明参粉末色度值,然后采用热浸法、硫酸-苯酚法以及超高效液相色谱法分别测定水溶性浸出物、醇溶性浸出物、多糖、欧前胡素等指标成分质量分数,最后使用IBM SPSS Statistics 26.0软件分析色度值与各指标成分间的相关性。[结果]川明参粉末色度值L*与醇溶性浸出物、水溶性浸出物以及欧前胡素质量分数呈极显著负相关(P<0.01),与多糖质量分数呈显著负相关(P<0.05);ΔE*与水溶性浸出物及欧前胡素质量分数呈极显著负相关(P<0.01),与醇溶性浸出物、多糖质量分数呈显著负相关(P<0.05);a*、b*与醇溶性浸出物、水溶性浸出物、欧前胡素质量分数呈极显著正相关(P<0.01),与多糖质量分数呈显著正相关。[结论]色度值可作为快速判断评价川明参药材质量的指标,为全面评价川明参药材品质提供科学依据。[Objective]Explored the correlation between color and several index components of Chuanminshen violaceum Sheh et Shan,aiming at providing a reference for rapid and efficient screening of high quality Chuanminshen violaceum.[Method]Spectrophotometer was used to determine the color value of Chuanminshen violaceum;hot leaching method,sulfuric acid-phenol method,ultra-high performance liquid chromatography method were used to determine watersoluble extract,alcohol-soluble extract,polysaccharide and imperatorin respectively.IBM SPSS Statistics 26.0 was used to analyze the correlation between color and index component.[Result]The value of L*had extremely significant negative-correlation with alcohol-soluble extract,water-soluble extract and imperatorin(P<0.01),and significant negative-correlation with polysaccharide(P<0.05).The value ofΔE*had extremely significant negative correlation with water-soluble extract and imperatorin(P<0.01),and significant negative correlation with alcohol-soluble extract and polysaccharide(P<0.05).However,the values of a*and b*had extremely significant positive correlation with alcoholsoluble extract,water-soluble extract and imperatorin(P<0.01),and significant positive correlation with polysaccharide(P<0.05)[.Conclusion]The color values can be used as the index for rapid identification of quality of Chuanminshen vio⁃laceum,which provided scientific basis for comprehensive assessment of the quality of Chuanminshen violaceum.

关 键 词：川明参 色泽 浸出物 多糖含量 欧前胡素 质量评价 

分 类 号：R284[医药卫生—中药学]