血清mi R939-和mi R15b-表达与糖尿病视网膜病变患者微血管损伤的相关性分析  

Correlation analysis of serum miR-939 and miR-15b expression and microvascular injury in diabetic retinopathy patients

作　　者：王雅清 李红敏 张茜玉 王莉 王勇[1] 刘永胜[1] 庞英杰[1] WANG Yaqing;LI Hongmin;ZHANG Xiyu;WANG Li;WANG Yong;LIU Yongsheng;PANG Yingjie(Department of Endocrinology,Baoding Second Hospital,Baoding,Hebei 071000,China;Department of Endocrinology,the Seventh People′s Hospital of Hebei Province,Dingzhou,Hebei 073000,China;Department of Endocrinology,Tangshan Nanhu Hospital,Tangshan,Hebei 063013,China;Department of Endocrinology,Baoding Fifth Hospital,Baoding,Hebei 071000,China)

机构地区：[1]保定市第二医院内分泌科,河北保定071000 [2]河北省第七人民医院内分泌科,河北定州073000 [3]唐山南湖医院内分泌科,河北唐山063013 [4]保定市第五医院内分泌科,河北保定071000

出　　处：《国际检验医学杂志》2024年第4期471-475,共5页International Journal of Laboratory Medicine

基　　金：保定市科学技术研究与发展计划项目(2141ZF008)。

摘　　要：目的 分析血清miR-939和miR-15b表达与糖尿病视网膜病变(DR)患者微血管损伤的相关性。方法 选取2021年1月至2022年10月在保定市第二医院进行诊治的2型糖尿病患者176例作为研究对象。根据研究对象根据是否发生DR及病变程度分为无DR患者74例(NDR组)、非增殖型DR患者62例(NPDR组)、增殖型DR(PDR)患者40例(PDR组)。采用实时荧光定量PCR检测各组血清miR-939、miR-15b相对表达水平,酶联免疫吸附试验法检测各组血管内皮生长因子(VEGF)水平,流式细胞术检测内皮细胞(ECs)、内皮祖细胞(EPCs)和循环祖细胞(CPCs)计数百分比。比较3组患者血清miR-939、miR-15b、VEGF水平和ECs、EPCs、CPCs。采用Pearson相关性分析血清miR-939、miR-15b与VEGF、ECs、EPCs、CPCs的相关性。采用多因素Logistic回归分析2型糖尿病患者发生DR的影响因素。结果 PDR组、NPDR组血清miR-939、miR-15b相对表达水平低于NDR组,而血清VEGF水平高于NDR组,差异有统计学意义(P<0.05)。PDR组、NPDR组ECs高于NDR组,而EPCs和CPCs低于NDR组,差异有统计学意义(P<0.05)。血清miR-939与VEGF、ECs呈负相关(r=-0.407、-0.613,P<0.05),与EPCs、CPCs呈正相关(r=0.481、0.486,P<0.05),血清miR-15b与VEGF、ECs呈负相关(r=-0.539、-0.625,P<0.05),与EPCs、CPCs呈正相关(r=0.451、0.483,P<0.05)。多因素Logistic回归分析结果显示,2型糖尿病病程、糖化血红蛋白、餐后2 h血糖、VEGF、miR-939和miR-15b为2型糖尿病患者发生DR的影响因素(P<0.05)。结论 DR患者血清miR-939、miR-15b表达与VEGF、ECs、EPCs和CPCs密切相关,DR患者血清miR-939、miR-15b表达能够为其微血管损伤程度的早期判断与评估提供一定参考。Objective To analyze the correlation between serum miR-939 and miR-15b expression and microvascular injury in patients with diabetic retinopathy(DR).Methods A total of 176 patients with type 2 diabetes diagnosed and treated in the Baoding Second Hospital from January 2021 to October 2022 were selected as the study objects.The subjects were divided into 74 patients without DR(NDR group),62 patients with non-proliferative DR(NPDR group)and 40 patients with proliferative DR(PDR group)according to whether or not DR occurred and the degree of lesions.Real-time fluorescent quantitative PCR was used to detect the relative expression levels of miR-939 and miR-15b in serum of all groups,the level of vascular endothelial growth factor(VEGF)was detected by enzyme-linked immunosorbent assay,and the count percentage of endothelial cells(ECs),endothelial progenitor cells(EPCs)and circulating progenitor cells(CPCs)was detected by flow cytometry.Serum levels of miR-939,miR-15b,VEGF and ECs,EPCs and CPCs were compared in 3 groups.Pearson correlation was used to analyze the correlation between serum miR-939 and miR-15b and VEGF,ECs,EPCs and CPCs.Multivariate Logistic regression was used to analyze the factors affecting the occurrence of DR in patients with type 2 diabetes.Results The relative expression levels of miR-939 and miR-15b in PDR group and NPDR group were lower than those in NDR group,while the serum VEGF levels were higher than those in NDR group,with statistical significance(P<0.05).ECs in PDR group and NPDR group were higher than those in NDR group,while EPCs and CPCs were lower than those in NDR group,the difference was statistically significant(P<0.05).Serum miR-939 was negatively correlated with VEGF and ECs(r=-0.407,-0.613,P<0.05),and positively correlated with EPCs and CPCs(r=0.481,0.486,P<0.05).Serum miR-15b was negatively correlated with VEGF and ECs(r=-0.539,-0.625,P<0.05),and positively correlated with EPCs and CPCs(r=0.451,0.483,P<0.05).Multivariate Logistic regression analysis showed that the duration of ty

关 键 词：糖尿病视网膜病变 微血管损伤 miR-939 miR-15b 

分 类 号：R587.2[医药卫生—内分泌]