Identification and fine mapping of qSW2 for leaf slow wilting in soybean  

作　　者：Shengyou Li Changling Wang Chunjuan Yan Xugang Sun Lijun Zhang Yongqiang Cao Wenbin Wang Shuhong Song 

机构地区：[1]Institute of Crop Research,Liaoning Academy of Agricultural Sciences,Shenyang 110161,Liaoning,China

出　　处：《The Crop Journal》2024年第1期244-251,共8页作物学报（英文版）

基　　金：The study was supported by the National Natural Science Foundation of China(32101795,32301782);National Key Research and Development Program of China(2016YFD0100201-01);Liaoning Provincial Major Special Project of Agricultural Science and Technology(2022JH1/10200002,2021JH1/10400038);Key Research and Development Plan of Liaoning Science and Technology Department(2021JH2/1020027);Shenyang Seed Industry Innovation Project(22-318-2-12).

摘　　要：Drought is one of the abiotic stresses limiting the production of soybean(Glycine max).Elucidation of the genetic and molecular basis of the slow-wilting(SW)trait of this crop offers the prospect of its genetic improvement.A panel of 188 accessions and a set of recombinant inbred lines produced from a cross between cultivars Liaodou 14 and Liaodou 21 were used to identify quantitative-trait loci(QTL)associated with SW.Plants were genotyped by Specific-locus amplified fragment sequencing and seedling leaf wilting was assessed under three water-stress treatments.A genome-wide association study identified 26 SW-associated single-nucleotide polymorphisms(SNPs),including three located in a 248-kb linkage-disequilibrium(LD)block on chromosome 2.Linkage mapping revealed a major-effect QTL,qSW2,associated with all three treatments and adjacent to the LD block.Fine mapping in a BC_(2)F_(3) population derived from a backcross between Liaodou 21 and R26 confined qSW2 to a 60-kb interval.Gene expression and sequence variation analysis identified the gene Glyma.02 g218100,encoding an auxin transcription factor,as a candidate gene for qSW2.Our results will contribute significantly to improving drought-resistant soybean cultivars by providing genetic information and resources.

关 键 词：Drought GWAS Linkage mapping Slow wilting Soybean(Glycine max) 

分 类 号：S565.1[农业科学—作物学]