Combined ATAC-seq,RNA-seq,and GWAS analysis reveals glycogen metabolism regulatory network in Jinjiang oyster(Crassostrea ariakensis)  

作　　者：Biao Wu Xi Chen Jie Hu Zhen-Yuan Wang Yan Wang Da-You Xu Hao-Bing Guo Chang-Wei Shao Li-Qing Zhou Xiu-Jun Sun Tao Yu Xiao-Mei Wang Yan-Xin Zheng Guang-Yi Fan Zhi-Hong Liu 

机构地区：[1]State Key Laboratory of Mariculture Biobreeding and Sustainable Goods,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao,Shandong 266071,China [2]Laboratory for Marine Fisheries Science and Food Production Processes,Laoshan Laboratory,Qingdao,Shandong 266071,China [3]BGI-Qingdao,BGI-Shenzhen,Qingdao,Shandong 266426,China [4]National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China [5]Changdao Enhancement and Experiment Station,Chinese Academy of Fishery Sciences,Yantai,Shandong 265800,China [6]State Key Laboratory of Agricultural Genomics,BGI-Shenzhen,Shenzhen,Guangdong 518083,China

出　　处：《Zoological Research》2024年第1期201-214,共14页动物学研究（英文）

基　　金：supported by the National Key R&D Program of China(2022YFD2400105,2018YFD0900104);Central Publicinterest Scientific Institution Basal Research Fund,CAFS(2021XT0102,2023TD30);Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)(2021QNLM050103);Key Research and Development Project of Shandong Province(2021LZGC028);National Marine Genetic Resource Center。

摘　　要：Glycogen serves as the principal energy reserve for metabolic processes in aquatic shellfish and substantially contributes to the flavor and quality of oysters.The Jinjiang oyster(Crassostrea ariakensis)is an economically and ecologically important species in China.In the present study,RNA sequencing(RNA-seq)and assay for transposase-accessible chromatin using sequencing(ATAC-seq)were performed to investigate gene expression and chromatin accessibility variations in oysters with different glycogen contents.Analysis identified 9483 differentially expressed genes(DEGs)and 7215 genes with significantly differential chromatin accessibility(DCAGs)were obtained,with an overlap of 2600 genes between them.Notably,a significant proportion of these genes were enriched in pathways related to glycogen metabolism,including“Glycogen metabolic process”and“Starch and sucrose metabolism”.In addition,genome-wide association study(GWAS)identified 526 single nucleotide polymorphism(SNP)loci associated with glycogen content.These loci corresponded to 241 genes,63 of which were categorized as both DEGs and DCAGs.This study enriches basic research data and provides insights into the molecular mechanisms underlying the regulation of glycogen metabolism in C.ariakensis.

关 键 词：Crassostrea ariakensis GLYCOGEN TRANSCRIPTOME ATAC GWAS 

分 类 号：Q953[生物学—动物学]