基于UPLC指纹图谱和含量测定的不同产地密蒙花药材质量评价研究  

Study on quality evaluation of Buddleja officinalis from different habitats based on HPLC fingerprint and content determination

作　　者：位翠杰 刘晓霞 段志文 冯涌微 贾小舟 梁月仪 陈向东 孙冬梅 李振雨 Wei Cuijie;Liu Xiaoxia;Duan Zhiwen;Feng Yongwei;Jia Xiaozhou;Liang Yueyi;Chen Xiangdong;Sun Dongmei;Li Zhenyu(Guangdong Yifang Pharmaceutical Co.Ltd.,Key Laboratory of Traditional Chinese Medicine Formula of Guangdong Provincial,Foshan 528244,China;The First Affiliated Hospital of Guangdong University of TCM,Guangzhou 510400,China)

机构地区：[1]广东一方制药有限公司广东省中药配方颗粒企业重点实验室,佛山528244 [2]广州中医药大学附属第一医院,广州510400

出　　处：《国际中医中药杂志》2024年第2期215-221,共7页International Journal of Traditional Chinese Medicine

基　　金：国家工业和信息化部2019年产业技术基础公共服务平台项目(2019-00902-1-2)。

摘　　要：目的建立密蒙花超高效液相色谱(UPLC)指纹图谱和2个有效成分含量测定方法, 为全面评价不同产地密蒙花药材的质量提供参考。方法采用UPLC法建立密蒙花药材的指纹图谱;对其进行相似度评价、聚类分析、主成分分析和正交偏最小二乘法判别分析(OPLS-DA), 并测定密蒙花药材中毛蕊花糖苷和蒙花苷含量。结果 17批密蒙花药材指纹图谱有12个共有指纹峰, 经对照品比对, 指认其中6个指纹峰, 分别为松果菊苷、蒙花苷、木犀草苷、毛蕊花糖苷、异类叶升麻苷、芹菜素;17批密蒙花药材指纹图谱相似度均大于0.9;不同产地的药材分为2类, 采用OPLS-DA发现5个差异性标志物, 显著性差异排序分别为毛蕊花糖苷>峰3>松果菊苷>异类叶升麻苷>蒙花苷;采用指纹图谱方法对伪品结香花药材进行有效鉴别;湖北和四川产区的密蒙花药材含量较高且稳定。结论该方法可有效分析不同产地密蒙花药材的质量差异, 为其质量控制提供依据。Objective To establish UPLC fingerprint method and 2 contents determination methods of Buddleja officinalis;To provide a reference for improving the quality control standard and evaluation of Buddleja officinalis from different habitats.Methods UPLC method was used to establish the fingerprints of 17 batches of Buddleja officinalis.The similarity evaluation,clustering analysis,principal component analysis and orthogonal partial least squares discriminant analysis were used to compare the quality differences of Buddleja officinalis from different habitats.The contents of acteoside and linarin in Buddleja officinalis were determined.Results There were 12 common peaks in UPLC fingerprints of Buddleja officinalis,six of which were identified as echinacoside,acteoside,cynaroside,isoacteoside,linarin,and apigenin.The fingerprint similarity of 17 batches of Buddleja officinalis was more than 0.9;Buddleja officinalis from different habitats were classified into 2 groups.Five differential markers were determined by OPLS-DA analysis.The order of significance was acteoside>peak 3>echinacoside>isoacteoside>linarin.Edgeworthia chrysantha was identified by the method of fingerprint as counterfeit.The results of content determination showed that the content of Buddleja officinalis in Hubei and Sichuan was the high and stable.Conclusion The method can effectively analyze the differences of Buddleja officinalis from different habitats,and provide reference for the quality control of Buddleja officinalis.

关 键 词：密蒙花 UPLC指纹图谱 含量测定(中药) 质量控制 

分 类 号：R284.1[医药卫生—中药学]