基于PI3K/Akt信号通路研究灯盏花乙素对子宫内膜癌Ishikawa细胞的影响  

Effects of scutellarin on endometrial carcinoma Ishikawa cells based on PI3K/Akt signaling pathway

作　　者：代丽丽[1] 王景 魏许瑞 马庆亚 王娜[2] 都治香[2] DAI Li-li;WANG Jing;WEI Xu-rui;MA Qing-ya;WANG Na;DU Zhi-xiang(Department of Obstetrics,the First Hospital of Hebei Medical University,Shijiazhuang 050000,Hebei Province;Department of Gynecology,the First Hospital of Hebei Medical University,Shijiazhuang 050000,Hebei Province,China)

机构地区：[1]河北医科大学第一医院产科,河北石家庄050000 [2]河北医科大学第一医院妇科,河北石家庄050000

出　　处：《中国临床药理学杂志》2024年第1期27-31,共5页The Chinese Journal of Clinical Pharmacology

基　　金：河北省医学科学课题研究计划基金资助项目(20210081)。

摘　　要：目的研究灯盏花乙素对子宫内膜癌Ishikawa细胞的影响,并分析与磷脂酰肌醇3激酶(PI3K)/蛋白激酶B(Akt)信号通路的相关性。方法将Ishikawa细胞分为空白组及低、中、高剂量实验组,分别用含0、5、10及20μmol·L-1的灯盏花乙素的完全培养基处理。用细胞计数试剂盒-8(CCK-8)实验检测细胞活力;用平板克隆实验检克隆形成能力;用划痕及Transwell实验检测转移及侵袭能力;用流式细胞术检测凋亡;用蛋白质印迹法检测蛋白表达情况。结果空白组和低、中、高剂量实验组48 h的细胞活力分别为(100.00±0.00)%、(78.51±7.54)%、(52.93±4.91)%和(41.62±5.33)%;克隆形成率分别为(100.00±0.00)%、(56.59±6.34)%、(35.23±4.62)%和(10.66±1.91)%;划痕愈合率分别为(53.70±6.19)%、(40.59±4.75)%、(34.25±4.40)%和(15.78±2.14)%;侵袭细胞数分别为(189.70±14.06)、(106.82±12.67)、(84.37±8.13)和(53.74±6.78)个;基质金属蛋白酶-2(MMP-2)蛋白相对表达水平分别为0.96±0.10、0.73±0.06、0.68±0.08和0.42±0.05;MMP抑制药-1(TIMP-1)蛋白相对表达水平分别为0.35±0.04、0.51±0.05、0.74±0.08和1.20±0.14;细胞凋亡率分别为(4.21±0.53)%、(15.83±2.42)%、(22.72±3.85)%和(34.41±4.67)%;B淋巴细胞瘤-2(Bcl-2)蛋白表达水平分别为1.38±0.15、0.90±0.10、0.56±0.06和0.24±0.03;Bcl-2相关X蛋白(Bax)表达水平分别为0.31±0.02、0.44±0.04、0.93±0.11和1.26±0.14;PI3Kp85蛋白表达水平分别为0.67±0.05、0.42±0.04、0.36±0.02和0.28±0.03;磷酸化Akt(p-Akt)蛋白表达水平分别为0.78±0.06、0.53±0.04、0.46±0.05和0.42±0.03;低、中、高剂量实验组上述指标与空白组比较,差异均有统计学意义(P<0.05或P<0.01)。结论灯盏花乙素可以通过调控PI3K/Akt信号通路抑制Ishikawa细胞增殖、转移及侵袭,并诱导其凋亡。Objective To study the effects of scutellarin on endometrial carcinoma Ishikawa cells,and analyze the correlation between the effects and phosphatidyl inositol 3 kinase(PI3K)/protein kinase B(Akt)signaling pathway.Methods Ishikawa cells were divided into blank group and experimental-L,-M,-H groups,each group was treated with complete medium containing 0,5,10 and 20μmol·L-1 scutellarin,respectively.Cell viability,clonal formation ability,metastatic ability,invasion,apoptosis and protein expression were detected by cell counting kit-8(CCK-8),plate cloning,scratch,Tra ns well,flow cytometry and Western blot assay,respectively.Results The cell viability of blank group and experimental-L,-M,-H groups at 48 h were(100.00±0.00)%,(78.51±7.54)%,(52.93±4.91)%and(41.62±5.33)%;the clone formation rate were(100.00±0.00)%,(56.59±6.34)%,(35.23±4.62)%and(10.66±1.91)%;the scratch healing rate were(53.70±6.19)%,(40.59±4.75)%,(34.25±4.40)%and(15.78±2.14)%;the number of invasive cells were 189.70±14.06,106.82±12.67,84.37±8.13 and 53.74±6.78;the relative expression levels of matrixmetallo proteinase-2 were 0.96±0.10,0.73±0.06,0.68±0.08 and 0.42±0.05;tissue inhibitor of MMP-1(TIMP-1)were 0.35±0.04,0.51±0.05,0.74±0.08 and 1.20±0.14;the apoptosis rates were(4.21±0.53)%,(15.83±2.42)%,(22.72±3.85)%and(34.41±4.67)%;the relative expression levels of B cell lymphoma-2(Bcl-2)were 1.38±0.15,0.90±0.10,0.56±0.06 and 0.24±0.03;Bcl-2 associated X protein(Bax)were 0.31±0.02,0.44±0.04,0.93±0.11 and 1.26±0.14;the relative expression levels of PI3Kp85 were 0.67±0.05,0.42±0.04,0.36±0.02 and 0.28±0.03;phosphorylated Akt(p-Akt)were 0.78±0.06,0.53±0.04,0.46±0.05 and 0.42±0.03.Compared with the blank group,the above indexes in the experimental-L,-M,-H groups were statistically significant(P<0.05 or P<0.01).Conclusion Scutellarin can inhibit the proliferation,metastatic ability and invasion of endometrial carcinoma Ishikawa cells and promote apoptosis by regulating PI3K/Akt signaling pathway.

关 键 词：灯盏花乙素 子宫内膜癌 转移 侵袭 凋亡 

分 类 号：R28[医药卫生—中药学]