三七多糖体内抗疲劳研究  

Experimental study on anti-fatigue effect of Polysaccharides of Panax notoginseng

作　　者：位盼盼 晏子俊 邓萌玥 夏谍 丁俞珍 张磊 陈彤[1] WEI Pan-pan;YAN Zi-jun;DENG Meng-yue;XIA Die;DING Yu-zhen;ZHANG Lei;CHEN Tong(School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products,Kunming Medical University,Kunming 650500,Yunnan Province,China;Department of Ultrasound Medicine,The Third Affiliated Hospital of Kunming Medical University,Kunming 650118,Yunnan Province,China)

机构地区：[1]昆明医科大学药学院暨云南省天然药物药理重点实验室,云南昆明650500 [2]昆明医科大学第三附属医院超声医学科,云南昆明650118

出　　处：《中国临床药理学杂志》2024年第1期87-91,共5页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金地区科学基金资助项目(81760698);第七批云南省高校工程研究中心立项建设基金资助项目([2019]-57)。

摘　　要：目的探究三七多糖(PPN)对小鼠抗运动疲劳的作用。方法将100只KM小鼠随机分为阴性对照组、阳性对照组和低、中、高剂量实验组,每组20只。低、中、高剂量实验组分别给予100、200、400 mg·kg^(-1)PPN;阳性对照组给予200 mg·kg^(-1)抗坏血酸;阴性对照组给予0.1 mL·10 g^(-1)0.9%NaCl。5组小鼠每天灌胃给药1次,连续28 d。末次给药后,分别测定负重游泳时间;小鼠非负重游泳90 min后,休息30 min,检测乳酸(LD)、血尿素氮(BUN)、糖原和丙二醛(MDA)水平;以脏器指数和组织病理学检测PPN的安全性。结果阴性对照组、阳性对照组和低、中、高剂量实验组小鼠体内LD水平分别为(4.76±0.84)、(2.86±0.34)、(3.00±0.69)、(2.35±0.65)、(1.39±0.48)mg·kg^(-1),BUN含量分别为(13.65±1.25)、(12.55±0.91)、(12.12±1.24)、(11.06±1.30)、(9.85±1.05)mmol·L^(-1),肝糖原含量分别为(3.24±0.56)、(11.11±2.16)、(5.61±1.41)、(6.60±1.49)、(12.05±2.25)mg·g^(-1),MDA水平分别为(2.36±0.21)、(1.23±0.41)、(1.93±0.23)、(1.73±0.21)、(1.04±0.18)mg prot·mL^(-1)。阳性对照组和低、中、高剂量实验组的上述指标与阴性对照组比较,在统计学上差异均有统计学意义(P<0.05,P<0.01,P<0.001)。结论PPN可以增加小鼠运动耐力,具有抗疲劳作用,本研究为PPN在抗疲劳研究领域的应用提供了一定的理论基础。Objective To explore the effect of Polysaccharides of Panax notoginseng(PPN)on anti-exercise fatigue in mice.Methods One hundred male KM mice were randomly divided into negative control group,positive control group and experimental-L,-M,-H groups,with 20 cases per group.Experimental-L,-M,-H groups was given 100,200,400 mg·kg^(-1)PPN,respectively;positive control group was given 200 mg·kg^(-1)vitamin C;negative control group was given 0.1 mL·10 g^(-1)0.9%NaCl.Five groups were gavaged once a day for 28 days.After the last administration,the loaded swimming time was measured;after 90 minutes of the unloaded swimming test,the mice were allowed to rest for 30 minutes,the levels of lactic acid(LD),blood urea nitrogen(BUN),glycogen,and malondialdehyde(MDA)were measured,the safety of PPN with organ indices and histopathology.Results LD levels in negative control group,positive control group and experimental-L,-M,-H groups were(4.76±0.84),(2.86±0.34),(3.00±0.69),(2.35±0.65)and(1.39±0.48)mg·kg^(-1);BUN contents were(13.65±1.25),(12.55±0.91),(12.12±1.24),(11.06±1.30)and(9.85±1.05)mmol·L^(-1);liver glycogen contents were(3.24±0.56),(11.11±2.16),(5.61±1.41),(6.60±1.49)and(12.05±2.25)mg·g^(-1);MDA levels were(2.36±0.21),(1.23±0.41),(1.93±0.23),(1.73±0.21)and(1.04±0.18)mg prot·mL^(-1).Compared with negative control group,the differences of above indexes in the positive control group and experimental-L,-M,-H groups were statistically significant(P<0.05,P<0.01,P<0.001).Conclusion PPN can increase exercise endurance in mice and has an anti-fatigue effect.This study provides a theoretical basis for the application of PPN in the field of anti-fatigue research.

关 键 词：三七多糖 运动疲劳 负重游泳 非负重游泳 抗疲劳 

分 类 号：R28[医药卫生—中药学]