B超监测梅花鹿性控精液腹腔镜输精试验  

The Insemination of Sexed Sperms by Laparoscopy Combined With the B-mode Ultrasonography in Sika Deer

作　　者：赵伟刚[1] 孟庆江[1] 魏海军[1] 陈秀敏[1] 杨镒峰[1] 赵蒙[1] 薛海龙[1] ZHAO Weigang;MENG Qingjiang;WEI Haijun;CHEN Xiumin;YANG Yifeng;ZHAO Meng;XUE Hailong(Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences,Changchun 130112,China)

机构地区：[1]中国农业科学院特产研究所,吉林长春130112

出　　处：《特产研究》2024年第1期13-16,23,共5页Special Wild Economic Animal and Plant Research

基　　金：吉林省科技发展计划项目(20210202127NC)。

摘　　要：梅花鹿常规冻精与性控冻精解冻后进行品质鉴定,结合B超监测卵泡有选择的对60只同期发情处理的梅花鹿进行腹腔镜精准输精。数据分析显示,同期发情处理后梅花鹿卵巢B超监测结果与腹腔镜输精受胎率存在明显的正相关;X和Y型冻精产仔率为83.33%,而常规冻精产仔率为65.31%,X、Y型冻精与常规冻精组间差异显著(P<0.05);常规冻精组、X冻精组与Y冻精组所产后代性别比(公/母)分别为9:8、0:10和16:1,X、Y型冻精与常规冻精组间差异显著(P<0.05),X、Y型冻精与常规冻精所产后代出生及60 d时的体重差异不显著(P <0.05)。结果表明,结合B超监测有选择的进行梅花鹿性控精液腹腔镜人工输精,可显著提高受胎率,降低成本,技术可行。Sexing sperm(X or Y) and routine sperm were assessed after thawed.They were evaluated and inseminated into 60 female sika deer which were treated with synchronous estrus,and combined with monitoring the follicular by B-mode ultrasonography.Data analysis showed that there was a significant positive correlation between B-mode ultrasonography monitoring results of sika deer ovary and laparoscopic insemination fertilization rate;the litter rate of X and Y-type frozen semen was 83.33%,while that of conventional frozen semen was65.31%,there were significant differences between the X,Y-type frozen semen and conventional frozen semen groups(P <0.05);the sex ratios of off spring(male/female) in conventional frozen semen,X and Y-type frozen semen were 9:8,0:10 and 16:1,respectively.The difference between the groups was significant(P <0.05),and the body weight of the offspring produced by the X,Y-type frozen semen and the conventional frozen semen at birth and 60 days was not significantly different(P<0.05).The results show that the selective laparoscopic artificial insemination of sika deer sex-controlled semen combined with B-mode ultrasonography monitoring can significantly improve the conception rate,reduce costs,and is technically feasible.

关 键 词：梅花鹿 流式细胞仪 精液分离 B超 腹腔镜 人工授精 

分 类 号：S825[农业科学—畜牧学]