两种富含甘氨酸的RNA结合蛋白参与选择性剪接实现对水稻耐热性的昼夜节律调控  

Diurnal regulation of alternative splicing associated with thermotolerance in rice by two glycine-rich RNA-binding proteins

作　　者：杨闯 罗安妮 芦海平 Seth Jon Davis 刘建祥 Chuang Yang;Anni Luo;Hai-Ping Lu;Seth Jon Davis;Jian-Xiang Liu(State Key Laboratory of Plant Environmental Resilience,College of Life Sciences,Zhejiang University,Hangzhou 310027,China;Department of Biology,University of York,York YO105DD,UK)

机构地区：[1]State Key Laboratory of Plant Environmental Resilience,College of Life Sciences,Zhejiang University,Hangzhou 310027,China [2]Department of Biology,University of York,York YO105DD,UK

出　　处：《Science Bulletin》2024年第1期59-71,共13页科学通报（英文版）

基　　金：the State Key Project of Research and Development Plan of China(2022YFF1001603 and2021YFF1000404);the Natural Science Foundation of Zhejiang,China(LD21C020001)。

摘　　要：Rice(Oryza sativa L.)production is threatened by global warming associated with extreme high temperatures,and rice heat sensitivity is differed when stress occurs between daytime and nighttime.However,the underlying molecular mechanism are largely unknown.We show here that two glycine-rich RNA binding proteins,Os GRP3 and Os GRP162,are required for thermotolerance in rice,especially at nighttime.The rhythmic expression of Os GRP3/Os GRP162 peaks at midnight,and at these coincident times,is increased by heat stress.This is largely dependent on the evening complex component Os ELF3-2.We next found that the double mutant of Os GRP3/Os GRP162 is strikingly more sensitive to heat stress in terms of survival rate and seed setting rate when comparing to the wild-type plants.Interestingly,the defect in thermotolerance is more evident when heat stress occurred in nighttime than that in daytime.Upon heat stress,the double mutant of Os GRP3/Os GRP162 displays globally reduced expression of heat-stress responsive genes,and increases of m RNA alternative splicing dominated by exon-skipping.This study thus reveals the important role of Os GRP3/Os GRP162 in thermotolerance in rice,and unravels the mechanism on how Os GRP3/Os GRP162 regulate thermotolerance in a diurnal manner.

关 键 词：Alternative splicing Diurnal regulation Glycine-rich protein Oryza sativa L. THERMOTOLERANCE 

分 类 号：S511[农业科学—作物学]