Artemisia argyi extract subfraction exerts an antifungal effect against dermatophytes by disrupting mitochondrial morphology and function  

作　　者：CHEN Le ZHU Yunyun GUO Chaowei GUO Yujie ZHAO Lu MIAO Yuhuan DU Hongzhi LIU Dahui 

机构地区：[1]College of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430065,China [2]National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China [3]College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China

出　　处：《Chinese Journal of Natural Medicines》2024年第1期47-61,共15页中国天然药物（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(No.32270391);the Natural Science Foundation of Hubei Province(Nos.2023AFA032 and 2022CFB391);the Young Qihuang Scholars of the State Administration of Traditional Chinese Medicine,Hubei Province Administration of Traditional Chinese Medicine Research Project(No.ZY2023Z023);the Earmarked Fund for CARS-21 and Key Project at Central Government Level:the Ability Establishment of Sustainable Use for Valuable Chinese Medicine Resources(No.2060302).

摘　　要：Artemisia argyi(A.argyi),a plant with a longstanding history as a raw material for traditional medicine and functional diets in Asia,has been used traditionally to bathe and soak feet for its disinfectant and itch-relieving properties.Despite its widespread use,scientific evidence validating the antifungal efficacy of A.argyi water extract(AAWE)against dermatophytes,particularly Trichophyton rubrum,Trichophyton mentagrophytes,and Microsporum gypseum,remains limited.This study aimed to substantiate the scientific basis of the folkloric use of A.argyi by evaluating the antifungal effects and the underlying molecular mechanisms of its active subfraction against dermatophytes.The results indicated that AAWE exhibited excellent antifungal effects against the three aforementioned dermatophyte species.The subfraction AAWE6,isolated using D101 macroporous resin,emerged as the most potent subfraction.The minimum inhibitory concentrations(MICs)of AAWE6 against T.rubrum,M.gypseum,and T.mentagrophytes were 312.5,312.5,and 625μg·mL−1,respectively.Transmission electron microscopy(TEM)results and assays of enzymes linked to cell wall integrity and cell membrane function indicated that AAWE6 could penetrate the external protective barrier of T.rubrum,creating breaches(“small holes”),and disrupt the internal mitochondrial structure(“granary”).Furthermore,transcriptome data,quantitative real-time PCR(RT-qPCR),and biochemical assays corroborated the severe disruption of mitochondrial function,evidenced by inhibited tricarboxylic acid(TCA)cycle and energy metabolism.Additionally,chemical characterization and molecular docking analyses identified flavonoids,primarily eupatilin(131.16±4.52 mg·g^(−1))and jaceosidin(4.17±0.18 mg·g^(−1)),as the active components of AAWE6.In conclusion,the subfraction AAWE6 from A.argyi exerts antifungal effects against dermatophytes by disrupting mitochondrial morphology and function.This research validates the traditional use of A.argyi and provides scientific support for its antider

关 键 词：Artemisia argyi DERMATOPHYTE Antifungal activity Mitochondrial structure Mitochondrial function Transcriptome analysis 

分 类 号：R965[医药卫生—药理学]