人工智能识别60 Coγ射线照射诱导人外周血微核的剂量⁃效应曲线建立  

Establishing a dose⁃response curve for 60Co γ⁃ray irradiation⁃induced micronuclei in human peripheral blood cells using artificial intelligence⁃based color recognition

作　　者：吴梦云 李炜[1] 张华东[1] 袁方[1] 谭秀洪[1] Wu Mengyun;Li Wei;Zhang Huadong;Yuan Fang;Tan Xiuhong(Radiology Laboratory,Department of Occupational Health and Radiological Health,Chongqing Center for Disease Control and Prevention,Chongqing 400707,China)

机构地区：[1]重庆市疾病预防控制中心公共卫生所职业与放射卫生放射检验科,重庆400707

出　　处：《中华放射医学与防护杂志》2024年第1期7-12,共6页Chinese Journal of Radiological Medicine and Protection

基　　金：重庆市自然科学基金(CSTB2022NSCQ⁃MSX1220);重庆市科卫联合医学科研项目(2022QNXM030,2022ZDXM034)。

摘　　要：目的根据扫描显微镜搭配玻片扫描软件(Metafer4),在松弛素B(CB)阻断微核法试验中识别和鉴定微核,建立^(60)Co γ射线照射剂量与人外周血淋巴细胞微核率的剂量-效应曲线。方法采集4名健康人(2男2女)肘静脉血样品,用0、0.25、0.5、1、2、3、4和5Gy ^(60)Co γ射线(剂量率0.74Gy/min)离体照射,胞质分裂阻断微核法培养、收获和制备标本玻片,人工智能彩色识别分析系统分析并记录双核细胞和微核数。应用CABAS软件拟合基于微核率的剂量-效应曲线。2份照射后的盲样进行生物剂量估算验证。结果在0~5Gy剂量范围内,拟合的微核剂量-效应曲线符合二次多项式模型,回归方程为y=0.0321D^(2)+0.0237D+0.0127(R^(2)=0.998,D为剂量)。用拟合曲线对验证样本的剂量估算结果与实际照射剂量基本接近。结论成功建立基于人工智能识别微核的剂量-效应曲线,为估算辐射生物剂量提供了可行方法。Objective To identify micronuclei through the cytochalasin B blocking micronucleus method⁃based assay using scanning microscope,combined with the slide scanning software Metafer 4 and,accordingly,to establish a dose⁃response relationship between the dose of ^(60)Co γ⁃rays and the frequency of micronuclei in human peripheral blood lymphocytes using artificial intelligence⁃based color recognition.Methods Blood samples were collected from four healthy individuals(two men and two women)and were then exposed to varying doses of 60Co γ⁃ray radiation(0,0.25,0.5,1,2,3,4,5 Gy)at a dosage rate of 0.74 Gy/min.Micronucleus slides were prepared as per the GBZ 128-2023 standard.The numbers of binuclear cells and micronuclei were recorded using an artificial intelligence⁃based color recognition analysis system.The dose⁃response curve was determined through fitting using the CABAS software.Then,the doses to both independent samples were estimated based on the curve.Results Within a dose range of 0 to 5 Gy,the fitted micronucleus dose⁃response curve aligned with a quadratic polynomial model,with a regression equation of y=0.0321D^(2)+0.0237 D+0.0127(D denoting the dose,correlation coefficient R^(2)=0.998).The dose estimations from the validation samples closely corresponded to the actual irradiation doses.Conclusions Establishing the micronucleus dose⁃response curve provides a feasible method and basis for the rapid and accurate estimation of radiation biological doses in laboratory automation.

关 键 词：人工智能 剂量-效应曲线 松弛素B阻断微核法 人外周血 

分 类 号：R144.1[医药卫生—公共卫生与预防医学]