基于SSR分子标记吉安地区的水稻亲缘关系鉴定分析  

IDENTIFICATION OF RICE VARIETIES BASED ON SSR MOLECULAR MARKERS IN JI’AN

作　　者：郑卓[1] 张天舒 李华 王莉莉 吴杨[1] 贺卫东 孙惠敏[1] ZHENG Zhuo;ZHANG Tian-shu;LI Hua;WANG Li-li;WU Yang;HE Wei-dong;SUN Hui-min(School of Life Sciences,Jinggangshan University,Ji’an,Jiangxi 343009,China;Qingyuan District Department of Agriculture,Ji’an,Jiangxi 343009,China;Health Science Center,Jinggangshan University,Ji’an,Jiangxi 343009,China)

机构地区：[1]井冈山大学生命科学学院,江西吉安343009 [2]吉安市青原区农业农村局,江西吉安343009 [3]井冈山大学医学部,江西吉安343009

出　　处：《井冈山大学学报（自然科学版）》2024年第1期48-53,共6页Journal of Jinggangshan University (Natural Science)

基　　金：国家自然科学基金项目(31860366);江西省教育厅科技计划项目(GJJ209927,GJJ2201626,GJJ160747)。

摘　　要：稻种亲缘关系分析是生产优质杂交水稻的必要条件。本研究选取均匀分布于水稻基因组12条染色体的SSR分子标记,对8份水稻样品进行SSR分子标记分析,通过聚类分析法将其归类并计算得出遗传相似系数后,对8份水稻样品的亲缘关系进行了鉴定。结果表明:所选取的8份水稻样品中,其相似系数为0.65~1.00,在遗传相似系数0.65处,8份水稻样品聚为两大类群,其中5号‘赣迟A04’单独为一类,其他水稻样品聚为一类。在遗传相似系数0.80处,可划分为三大类群:第一类囊括了6种样品,表明这6个品种亲缘关系较近,其中6号‘赣早A02’与7号‘吉安早A07’疑似同一样品,3号‘井冈山迟A03’与4号‘井冈山早A05’的亲缘关系最近,而6号、7号同时与8号‘吉安迟A09’保持有最近的亲缘关系;第二类只含有2号‘迟-2’样品株;第三类则仅有5号稻株‘赣迟A04’,说明其与其他7份水稻样品的亲缘关系最远。本研究结果为杂交水稻的应用提供重要信息。Analysis of genetic relationship of rice seed is a necessary condition for producing high quality hybrid rice.In this study,SSR molecular markers were selected from 12 chromosomes of rice genome and analyzed in 8 rice samples,the genetic similarity coefficient was calculated by cluster analysis to identify their genetic relationship.The results showed that the similarity coefficient of 8 rice samples was 0.65~1.00,and at the genetic similarity coefficient of 0.65,the 8 rice samples were clustered into two groups,among them,No.5'Ganchi A04'was classified as a single group,and the other samples were clustered into another single group.At the genetic similarity coefficient of 0.80,it can be divided into three groups.The first group includes six samples,which shows that the six varieties are closely related,no 6'Ganzao A02'and No 7'Ji'an Zao A07'are suspected to be the same samples,and No 3'Jinggang Mountains Zao A03'and no 4'Jinggang Mountains Zao A05'are the most closely related samples,while No.6 and No.7 were closely related to No.8'Ji'an chi A09',only No.2'Chi-2'was found in the second group,and only No.5'Gan Chi A04'was found in the third group,the results indicated that it had the farthest relationship with the other 7 rice samples.This study provide important information for the application of hybrid rice.

关 键 词：水稻 SSR分子标记 品种鉴定 聚类分析 

分 类 号：S330.25[农业科学—作物遗传育种] S511[农业科学—农艺学]