西藏湿生扁蕾根际土壤微生物群落结构特征分析  

Microbial community characteristics in rhizosphere soil of Gentianopsis paludosa in Tibet

作　　者：张二豪 达瓦秋宗 徐雨婷 尹秀[1] 禄亚洲[1] 蔡皓 ZHANG Er-hao;DAWA Qiu-zong;XU Yu-ting;YIN Xiu;LU Ya-zhou;CAI Hao(Tibet Agriculture and Animal Husbandry University,Linzhi,Tibet 860000,China)

机构地区：[1]西藏农牧学院,西藏林芝860000

出　　处：《西南农业学报》2023年第12期2741-2753,共13页Southwest China Journal of Agricultural Sciences

基　　金：西藏自治区自然科学基金项目(XZ202001ZR0063G);西藏农牧学院生物技术一流专业建设(2023-007)。

摘　　要：【目的】探究西藏不同产地湿生扁蕾根际土壤微生物群落组成特征,揭示根际土壤核心微生物菌群与根际土壤微生物群落的相互作用,以及影响微生物群落组成的环境因素。【方法】采用Illumina MiSeq高通量测序技术和生物信息学方法对西藏林芝市巴宜区(BY)、察隅县(CY)和工布江达县(GB)湿生扁蕾根际土壤微生物群落组成,及其与土壤理化因子间的相关性进行分析。【结果】湿生扁蕾根际土壤共获得4738个细菌OTUs和3600个真菌OTUs,BY样品根际土壤细菌和真菌丰富度指数最高,CY样品根际土壤细菌和真菌多样性指数最高;不同产地湿生扁蕾根际土壤细菌和真菌群落结构组成存在差异;根际土壤核心细菌菌群有322个属,核心真菌菌群有175个属;根际土壤细菌和真菌互作网络均具有模块化结构且以协同作用为主,核心微生物菌群相对分散,变形菌门、放线菌门、子囊菌门和担子菌门是根际土壤微生物的关键类群;相关性分析表明,核心微生物菌群的改变与土壤理化因子间存在不同程度的相关性;pH、总钾、总磷、总氮、速效钾和速效氮含量与根际土壤微生物群落结构组成显著正相关,总钾、总磷和总氮是影响根际土壤微生物α多样性的关键因子。【结论】不同产地湿生扁蕾根际土壤细菌和真菌群落组成及多样性存在一定的差异,根际土壤微生物菌群的改变与土壤理化因子间存在不同程度的相关性。本研究为湿生扁蕾人工种植及益生微生物菌群的筛选提供了理论依据。【Objective】The present study aimed to explore the microbial community characteristics in the rhizosphere soil of Gentianopsis palu⁃dosa from different regions in Tibet,and reveal the interaction between core microbiota and general microbiota,and explore the environmen⁃tal factors affecting composition of microbial community.【Method】The high⁃throughput sequencing technology and bioinformatics were used to analyze the composition of microbial community in rhizosphere soil of G.paludosa from Bayi(BY),Chayu(CY)and Gongbujiangda(GB)in Tibet,and the correlation between microbial community and physiochemical properties.【Result】A total of 4738 bacterial OTUs and 3600 fungal OTUs were obtained from rhizosphere soil of G.paludosa.The richness index of bacteria and fungi in BY sample was the highest,the diversity of bacteria and fungi in CY were the highest;The composition of the microbial community was different among different samples.The analysis of core microbial communities showed that there were 322 core bacterial genera and 175 fungal genera.Network analy⁃sis showed that the bacterial and fungal community in rhizosphere soil had modular structure and was dominated by positive correlation,and the core microbiota was relatively dispersed;Actinobacteriota,Proteobacteria,Ascomycota and Basidiomycota were the key microbial com⁃munity.The correlation analysis showed that the changes of microbial community in rhizosphere soil were correlated with the physiochemical properties,and the pH,total potassium,total phosphorus,total nitrogen,available potassium,and available nitrogen were significantly posi⁃tive correlation with microbial community,the total potassium,total phosphorus and total nitrogen were the key factors that affected the alpha diversity of microbial community in the rhizosphere soil of G.paludosa.【Conclusion】The composition and diversity of bacterial and fungal community were different among different samples,the changes of microbial community in rhizosphere soil were correlated with

关 键 词：湿生扁蕾 高通量测序 根际土壤微生物 土壤理化因子 核心微生物 

分 类 号：S154.3[农业科学—土壤学]