内蒙古地区手参根际土壤真菌群落组成及多样性  

Composition and diversity of fungi community in rhizosphere soil of Gymnadenia conopsea in Inner Mongolia

作　　者：阿拉坦存布尔 曹乌吉斯古楞 包金花[3] 查苏娜 胡红霞 王秀兰[1] Alatancunbuer;CAO Wujisiguleng;BAO Jin-hua;Chasuna;HU Hong-xia;WANG Xiu-lan(Medical College,Inner Mongolia Minzu University,Tongliao,Inner Mongolia 028000,China;Inner Mongolia Minzu University/Key Laboratory of Mongolian Medicine Research and Development Engineering,Ministry of Education,Tongliao,Inner Mongolia 028000,China;College of Agriculture,Inner Mongolia Minzu University,Tongliao,Inner Mongolia 028000,China)

机构地区：[1]内蒙古民族大学蒙医药学院,内蒙古通辽028000 [2]内蒙古民族大学/蒙医药研发工程教育部重点实验室,内蒙古通辽028000 [3]内蒙古民族大学农学院,内蒙古通辽028000

出　　处：《西南农业学报》2023年第12期2754-2762,共9页Southwest China Journal of Agricultural Sciences

基　　金：内蒙古自治区科技计划项目(2021GG0003);内蒙古民族大学博士启动基金项目(BS509)。

摘　　要：【目的】分析手参[Gymnadenia conopsea(L.)R.Br.]根际土壤真菌群落多样性及与土壤环境因子的相关性,为探究手参人工栽培、生态种植等资源保护及可持续利用提供参考。【方法】采用Illumina MiSeq高通量测序技术对内蒙古阿鲁科尔沁旗、东乌株穆沁旗和翁牛特旗手参根际土壤真菌群落的多样性进行高通量测序和分析,并对植株根际土壤理化性质与相关性进行分析。【结果】手参根际土壤呈酸性,富含有机质和速效钾,但少氮低磷,且不同分布区土壤理化性质差异显著。从3个分布区土壤样品中共获得5125个OTUs,隶属于11门、31纲、65目、161科、158属。在门水平,3个分布区手参根际土壤真菌的优势菌门均为子囊菌门(Ascomycota)和担子菌门(Basidiomycota)。在属水平,毛舌菌属(Trichoglossum)、湿伞属(Hygrocybe)、被孢霉属(Mortierella)为手参根际土壤真菌的优势类群。Alpha多样性指数表明,3个分布区手参根际土壤真菌群落多样性和丰富度总体趋势表现为阿鲁科尔沁旗(A1)>东乌珠穆沁旗(D3)>翁牛特旗(W2);Beta多样性指数表明,3个分布区手参的根际土壤真群落组成存在一定差异。土壤全钾、全磷、全氮和水分含量可能是影响内蒙古地区的手参根际土壤真菌群落组成的关键因子。湿伞属(Hygrocybe)和锁瑚菌属(Clavulinopsis)与土壤环境关键因子呈显著正相关;而具有致病特性的肉座菌属(Hypocrea)和镰刀菌属(Fusarium)与土壤环境关键因子呈显著负相关。【结论】明确了内蒙古地区分布的手参生长的土壤条件及根际土壤真菌群落特征,在3个分布区中,阿鲁科尔沁旗手参根际土壤真菌群落多样性和丰富度最高。土壤理化性质对手参根际土壤真菌多样性影响较大,其他影响因素需进一步研究。【Objective】The study aimed to analyze the diversity of fungi community in the rhizosphere soil of Gymnadenia conopsea and its correlation with soil environmental factors,so as to provide reference for exploring the work of resource protection and sustainable utilization such as artificial cultivation and ecological planting of G.conopsea.【Method】High⁃flux sequencing and comparative analysis were carried out on the diversity of rhizosphere soil fungal communities of G.conopsea in Aruhorqin Banner,Dongwuzhumuqin Banner and Ongniud Banner of Inner Mongolia by using Illumina Miseq high⁃throughput sequencing technology,and the physical and chemical properties and correlation of plant rhizosphere soil were analyzed.【Result】The rhizosphere soil of G.conopsea was acidic,rich in organic matter and available potassi⁃um,but low in nitrogen and phosphorus,and the soil physical and chemical properties in different distribution areas were significantly different.A total of 5125 OTUs were obtained from soil samples from three distribution areas,belonging to 11 phyla,31 classes,65 orders,161 families and 158 genera.At the phylum level,the dominant phyla of fungi in the rhizosphere soil of the three distribution areas were Ascomy⁃cota and Basidiomycota.At the genus level,Trichoglossum,Hygrocybe and Mortierella were the dominant groups of G.conopsea rhizosphere soil fungi.Alpha diversity index showed that the general trend of diversity and richness of rhizosphere soil fungal community in G.conopsea in the three distribution areas was A1>D3>W2;Beta diversity index showed that there were some differences in the composition of true communities in rhizosphere soil of G.conopsea in the three distribution areas.The contents of total potassium,total phosphorus,total nitro⁃gen and water in the soil may be the key factors affecting the composition of the fungal community in the rhizosphere soil of G.conopsea dis⁃tributed in Inner Mongolia.Among them,Hygrocybe and Clavulinosis had significant positive correlation wit

关 键 词：手参 根际土壤真菌 Alpha多样性 Beta多样性 冗余分析 

分 类 号：S154.39[农业科学—土壤学]