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作 者:胡清娥 HU Qing’e(Yunnan Management and Protection Bureau of the Upper Yangtze River Rare and Endemic Fish National Nature Reserve,Shuifu 657800,Hubei China)
机构地区:[1]长江上游珍稀特有鱼类国家级自然保护区云南管护局,湖北水富657800
出 处:《黑龙江水产》2024年第1期18-21,共4页Northern Chinese Fisheries
摘 要:研究探讨不同水平的艾叶(Artemisia argyi)粉对草鱼(Ctenopharyngodon idella)机体非特异性免疫力的影响,确定艾叶粉在草鱼饲料中的最适添加量。试验选择体重约为20g、体质健康的400尾草鱼随机分成4个处理组,每组4个重复,每个重复25尾草鱼。对照组草鱼投喂基础饲料不含有艾叶粉,试验A组草鱼饲喂含有2%的艾叶粉的基础饲料,试验B组草鱼饲喂含有4%的艾叶粉的基础饲料,试验C组草鱼饲喂含有8%的艾叶粉的基础饲料。饲喂60d后,检测各组草鱼机体的非特异性免疫指标。结果显示,试验B组和试验C组草鱼血清溶菌酶活力显著提高(P<0.05),试验B组和试验C组草鱼血清超氧化物歧化酶和谷胱甘肽过氧化物酶活力显著提高(P<0.05),所有艾叶粉组草鱼血清中丙二醛水平显著降低(P<0.05)。研究表明,草鱼饲料中添加适量的艾叶粉可以提高机体的非特异性免疫力,且以4%的添加量效果最佳。The study was conducted to investigate the effects of different levels of Artemisia argyi powder on the organismal non-specific immunity of grass carp(Ctenopharyngodon idella),and to determine the optimum amount of Artemisia argyi powder to be added to grass carp feed.In the experiment,400 grass carp weighing about 20g and in healthy condition were randomly divided into 4 treatment groups,with 4 replicates in each group and 25 fish in each replicate.The grass carp in the control group were fed a basal diet without ayahuasca,the grass carp in the test group A were fed a basal diet containing 2%ayahuasca,the grass carp in the test group B were fed a basal diet containing 4%ayahuasca,and the grass carp in the test group C were fed a basal diet containing 8%ayahuasca.After 60 d of feeding,the non-specific immunity indexes of the body of grass carp in each group were detected.The results showed that the serum lysozyme activity of grass carp in test group B and test group C was significantly increased(P<0.05),the serum superoxide dismutase and glutathione peroxidase activity of grass carp in test group B and test group C was significantly increased(P<0.05),and the serum malondialdehyde level of grass carp in all the aye-aye powder groups was significantly reduced(P<0.05).The study showed that the addition of appropriate amount of Artemisia absinthium powder to grass carp feed could improve the non-specific immunity of the organism,and the best effect was achieved with 4%of the addition.
关 键 词:艾叶(Artemisia argyi)粉 非特异性免疫 草鱼(Ctenopharyngodon idella)
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