一株耐碱好氧反硝化菌的分离鉴定及其脱氮性能  

Isolation and identification of an alkaline-tolerant aerobic denitrifying bacterium and its denitrification performance

作　　者：段兴帆 亢昕 赵毅彪 陈小华[2] 沈根祥[2] 赵晓祥[1] DUAN Xingfan;KANG Xin;ZHAO Yibiao;CHEN Xiaohua;SHEN Genxiang;ZHAO Xiaoxiang(College of Environmental Science and Engineering,Donghua University,Shanghai 201620,China;Shanghai Academy of Environmental Sciences,Shanghai 200233,China)

机构地区：[1]东华大学环境科学与工程学院,上海201620 [2]上海市环境科学研究院,上海200233

出　　处：《东华大学学报（自然科学版）》2024年第1期101-109,共9页Journal of Donghua University(Natural Science)

基　　金：上海市生态环境局科研项目(沪环科[2021]第10号)

摘　　要：为改善强碱环境下微生物脱氮效率低下的问题,从上海市稻田土壤中分离出一株具有强碱适应能力的好氧反硝化菌。经细胞形态学观察及16S rDNA分析,鉴定其为琼氏不动杆菌(Acinetobacter junii),并命名为琼氏不动杆菌5-2。结合单因素影响试验考察该菌株在不同环境条件下的脱氮效果,发现其在一定pH值(7.0~12.0)及盐质量浓度范围(10~30 g/L)内,均能保持较高的硝氮去除率(>90%)。在以乙酸钠为碳源、硝酸钾为氮源、碳氮质量比(m(C)/m(N))值为12、温度为35℃、转速为90 r/min、初始pH值为10.0、初始硝氮质量浓度为41.07 mg/L的条件下培养120 h后,该菌株对硝氮及总氮的去除率分别为97.83%及65.85%,同时,对该菌株好氧反硝化相关酶活性及基因进行检测。研究结果表明,琼氏不动杆菌5-2具有高效好氧反硝化能力,有望应用于处理实际含氮废水。To improve the low efficiency of microbial denitrification under strong alkaline environment,an aerobic denitrifying strain with strong alkaline adaptability was isolated from Shanghai paddy soil.The strain was identified as Acinetobacter junii by cell morphology and 16S rDNA analysis,and named as Acinetobacter junii strain 5-2.The effect of denitrification under different environmental conditions was investigated with single-factor effect experiments,and it was found that it could maintain a high nitrate removal rate(>90%)within a certain pH(7.0-12.0)and salt mass concentration range(10-30 g/L).After 120 h incubation with sodium acetate as carbon source,potassium nitrate as nitrogen source,m(C)/m(N)of 12,temperature of 35℃,rotation of 90 r/min,initial pH of 10.0 and initial nitrate-nitrogen content of 41.07 mg/L,the removal rates of nitrate-nitrogen and total nitrogen by this strain were 97.83%and 65.85%respectively.Meanwhile,the activity and genes of enzymes related to aerobic denitrification of this strain were examined.The results show that Acinetobacter junii strain 5-2 has high aerobic denitrification capacity and is expected to be applied to treat actual nitrogenous wastewater.

关 键 词：琼氏不动杆菌 废水处理 生物脱氮 好氧反硝化 耐碱 

分 类 号：X703[环境科学与工程—环境工程]