番木瓜环斑病毒甜瓜分离物的基因组及其侵染性克隆  

The genome and infectious clone of papaya ringspot virus melon isolate

作　　者：刘莉铭[1] 彭斌[1] 康保珊[1,2] 吴会杰 刘茜[1] 古勤生 LIU Liming;PENG Bin;KANG Baoshan;WU Huijie;LIU Xi;GU Qinsheng(Henan Key Laboratory of Fruit and Cucurbit Biology/Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,Henan,China;Zhongyuan Research Center,Chinese Academy of Agricultural Sciences,Xinxiang 453500,Henan,China)

机构地区：[1]河南省果树瓜类生物学重点实验室·中国农业科学院郑州果树研究所,郑州450009 [2]中国农业科学院中原研究中心,河南新乡453500

出　　处：《中国瓜菜》2024年第2期8-14,共7页China Cucurbits And Vegetables

基　　金：河南省科技攻关项目(202102110075);国家现代农业产业技术体系(CARS-25-2023-G20);中国农业科学院科技创新工程(CAAS-ASTIP-2022-ZFRI-09)。

摘　　要：番木瓜环斑病毒(papaya ringspot virus,PRSV)是瓜类作物主要病毒之一,分析了甜瓜分离物HaNHK10的基因组序列和分子变异,构建了具有侵染性的全长cDNA克隆。结果显示,HaNHK10分离物基因组全长为10332 nt,与其他分离物的核苷酸和氨基酸序列一致性分别为74.60%~97.80%和85.30%~98.50%。基于全基因组序列的系统进化分析显示,HaNHK10与来自中国的所有分离物均聚集于II组中,并与中国山东的西葫芦分离物PRSV-SD亲缘关系最近。接种试验显示,HaNHK10分离物的全长cDNA克隆具有侵染性,它能系统侵染甜瓜、黄瓜、西瓜、南瓜、西葫芦和瓠瓜6种作物,经接种产生的病毒后代也能够通过摩擦接种侵染植株。Papaya ringspot virus(PRSV)is one of the most important viruses in cucurbits crops.In this study,the genome sequence of melon isolate HaNHK10 was cloned and analyzed,and its full-length cDNA infectious clone was constructed.The results showed that the genome of isolate HaNHK10 was 10332 nt in length,and the identities of the genome nucleotide sequence and amino acid sequence between HaNHK10 and other isolates were 74.60%-97.80% and 85.30%-98.50%,respectively.Phylogenetic analysis based on genome sequences showed that HaNHK10 and all the isolates from China were clustered in group Ⅱ,and zucchini isolate PRSV-SD from Shandong province was most closely related to HaNHK10.The inoculation showed that the infectious clone was successfully constructed,and it could systematically infect melon,cucumber,watermelon,pumpkin,zucchini and bottle gourd.The progeny produced from the clone was infectious by mechanical inoculation.

关 键 词：番木瓜环斑病毒 甜瓜分离物 基因组 侵染性克隆 

分 类 号：S652[农业科学—果树学]