HIV/HCV双感患者病毒载量与生化指标研究  

Study on viral load and biochemical indexes of HIV/HCV co-infected patients

作　　者：冯鑫欢 张宁 张静静 齐杰 迪丽胡玛尔·艾萨江 陈志强 何晓燕 FENG Xin-huan;ZHANG Ning;ZHANG Jing-jing(The Second People's Hospital of Yining,Xinjiang Yining 835000)

机构地区：[1]伊宁市第二人民医院检验科,新疆维吾尔自治区伊宁835000 [2]伊宁市第二人民医院关爱中心,新疆维吾尔自治区伊宁835000

出　　处：《医学检验与临床》2024年第1期20-24,82,共6页Medical Laboratory Science and Clinics

基　　金：新疆艾滋病防控研究重点实验室开放课题,课题编号:XJYS1706-2021004。

摘　　要：目的:探讨HIV/HCV双感患者与HIV/AIDS患者病毒载量、生化指标之间的差异,以及与HIV单纯感染者HCV病毒载量(HCV-RNA)之间的关系,分析病毒载量与细胞免疫水平及生化指标间相关性,为HIV/HCV共感染的临床诊断、治疗提供依据。方法:收集伊宁市传染病医院2023年入院的HIV/AIDS感染者、HCV感染者、HIV/HCV双感患者血清样本,检测丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆红素(TBIL)等生化指标;采用流式细胞术对CD4+T淋巴细胞进行计数,采用PCR技术检测HCV-RNA及HIV-RNA。结果:共收集HIV/AIDS感染者、HCV感染者和HIV/HCV共感染者及正常人群共481例,其中HIV/AIDS感染组192例,HCV感染组162例,HIV/HCV共感染组111例,正常对照组16例。HIV/HCV组的HIV-RNA检出率(51.35%)高于HIV组(25.52%)(P<0.001),HCV-RNA检出率高于HCV组(P<0.001),CD4+T淋巴细胞水平低于HIV/AIDS感染组(P<0.001),ALT和AST均极显著高于对照组、HIV组、HCV组(P<0.001),HIV病毒载量与CD4+T淋巴细胞计数及ALT、AST、GGT水平未存在相关性(P>0.05),与CD4+T%存在负相关性(P<0.05),HCV病毒载量与ALT、AST、GGT水平均存在正相关性(P<0.05),而HIV病毒载量与HCV病毒载量间未存在相关性(P=0.145)。结论:HCV感染机体后,ALT、AST、GGT等肝功能指标异常增高,HIV/HCV共感染后机体免疫抑制加重,免疫功能更加低下,肝脏的损伤严重,机体对HIV及HCV病毒的复制控制能力下降。Objective:To explore the differences in viral load and biochemical indicators between HIV/HCV patients with dual infection and HIV/AIDS patients,as well as the relationship between HCV viral load and HCV viral load(HCV-RNA)in HIV simplex infection patients,and analyze the correlation between viral load and cellular immunity level and biochemical indicators,so as to provide evidence for clinical diagnosis and treatment of HIV/HCV co-infection.Methods:Serum samples of HIV/AIDS infected persons,HCV infected persons and HIV/HCV co-infected persons admitted to Yining Infectious Disease Hospital in 2023 were collected to detect alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBIL)and other biochemical indicators.CD4+T lymphocytes were counted by flow cytometry and HCV-RNA and HIV-RNA were detected by PCR.Results:A total of 481 cases of HIV/AIDS infected,HCV infected,HIV/HCV co-infected and normal population were collected,including 192 cases of HIV/AIDS infected group,162 cases of HCV infected group,111 cases of HIV/HCV co-infected group and 16 cases of normal control group.HIV-RNA detection rate was higher in HIV/HCV group(51.35%)than in HIV group(25.52%)(P<0.001),the detection rate of HCV-RNA was higher than that of HCV group(P<0.001),CD4+T lymphocyte level was lower than HIV/AIDS-infected group(P<0.001),ALT and AST were significantly higher than those in control group,HIV group and HCV group(P<0.001),there was no correlation between HIV viral load and CD4+T lymphocyte count,ALT,AST,GGT levels(P<0.05)was negatively correlated with CD4+T%(P<0.05).HCV viral load was positively correlated with ALT,AST and GGT levels(P<0.05),and there was no significant correlation between HIV viral load and HCV viral load(P=0.145).Conclusion:ALT,AST,GGT and other liver function indicators are abnormally increased after HIV/HCV co-infection,the body's immune suppression is aggravated,the immune function is even lower,the liver damage is serious,and the body's ability to control the replication of HIV and HCV

关 键 词：HIV/HCV 共感染 肝功能:HIV-RNA HCV-RNA 

分 类 号：R512.91[医药卫生—内科学] R512.63[医药卫生—临床医学]