单细胞转录组测序技术及其在免疫学研究中的应用  

Single-cell transcriptome sequencing and its application in immunology

作　　者：朱玉茹晨 李宇 王越[2,3] ZHU Yuruchen;LI Yu;WANG Yue(College of Basic Medical Sciences,Naval Medical University(Second Military Medical University),Shanghai 200433,China;Laboratory of Stem Cell and Regenerative Medicine,Center of Translational Medicine,Naval Medical University(Second Military Medical University),Shanghai 200433,China;Shanghai Key Laboratory of Cell Engineering,Shanghai 200433,China)

机构地区：[1]海军军医大学(第二军医大学)基础医学院,上海200433 [2]海军军医大学(第二军医大学)转化医学研究中心干细胞与再生医学研究室,上海200433 [3]上海市细胞工程重点实验室,上海200433

出　　处：《海军军医大学学报》2024年第2期145-154,共10页Academic Journal of Naval Medical University

基　　金：国家自然科学基金(82273317);上海市高等学校重点学科(四类)建设项目;上海市细胞工程重点实验室项目(14DZ2272300).

摘　　要：免疫系统具有复杂性、多变性以及可塑性,目前其组成和动态变化的研究只局限在群体效应,无法体现其异质性。单细胞技术的出现革新了研究者们对免疫学问题的认识。单细胞RNA测序技术是目前应用最广泛的单细胞技术,它能够在单细胞和单分子的水平进行免疫学分析,揭示生理或病理条件下的免疫反应过程。本文对单细胞RNA测序技术的一般流程、发展及其在免疫学方面的应用进展进行综述,并展望其在免疫学方面的发展前景。The immune system is characterized by complexity,diversity,and plasticity.Current research on the composition and dynamic changes of the immune system is limited to its population effects,and cannot fully reflect its heterogeneity.The emergence of single-cell technology has revolutionized our understanding of immunology.Single-cell RNA sequencing(scRNA-seq)is currently the most widely used single-cell technology,capable of analyzing a single cell or molecule to reveal the immune response under physiological or pathological conditions.This article provides a comprehensive review of the general process,development,and application of scRNA-seq in immunology,and discusses its future development in immunology.

关 键 词：免疫系统 单细胞测序 RNA测序 免疫反应 

分 类 号：R392[医药卫生—免疫学]