不同缺氧类型对小鼠血红蛋白性质的影响  

Effects of Different Types of Hypoxia on Hemoglobin Properties in Mice

作　　者：孙笑语 张岩冰 陈思漫 赵峥莹 王红霞[3] SUN Xiaoyu;ZHANG Yanbing;CHEN Siman;ZHAO Zhengying;WANG Hongxia(Experimental Teaching Center,School of Basic Medical Sciences,Capital Medical University,Beijing 100069,China;22019 Five-Year Stomatology Class,Capital Medical University,Beijing 100069,China;32019 Five-Year Pediatrics Class,Capital Medical University,Beijing 100069,China;42019 Five-Year Clinical Specialty Class,Capital Medical University,Beijing 100069,China;Department of Physiology and Pathophysiology,School of Basic Medical Sciences,Capital Medical University,Beijing 100069,China)

机构地区：[1]首都医科大学基础医学院实验教学中心,北京100069 [2]首都医科大学,北京100069 [3]首都医科大学基础医学院生理与病理生理学系,北京100069

出　　处：《继续医学教育》2024年第1期89-92,共4页Continuing Medical Education

基　　金：首都医科大学第二课堂项目(D2KT2021001)。

摘　　要：目的为了让学生更好地了解各类型缺氧所具有的特征,本实验不仅需要对各类型缺氧导致的小鼠呼吸、皮肤黏膜以及血液的颜色变化进行观察,还需检测不同类型缺氧时小鼠血红蛋白性质的改变。方法将小鼠随机分为正常对照组、密闭容器组、一氧化碳组和亚硝酸钠组,使用血氧分析仪测定小鼠血液中不同类型血红蛋白的百分比含量。研究时间为2021年2月至2022年5月。结果与正常对照组相比,密闭容器组小鼠还原性血红蛋白明显增高,一氧化碳组小鼠碳氧血红蛋白明显增高,腹腔注射亚硝酸钠组小鼠高铁血红蛋白明显增高,而4组小鼠总血红蛋白比较差异无统计学意义(P>0.05)。结论本研究通过观察不同原因复制的缺氧类型中血红蛋白性质的变化,从而明确缺氧模型实验中观察到的现象之间是否存在定量关系,提高实验的准确性。Objective In order to enhance students'understanding of the characteristics of various types of hypoxia,the experiment not only needs to observe the changes in mouse respiration,skin mucosa and blood color caused by different types of hypoxia,but also needs to detect the changes in the properties of hemoglobin in mice under different types of hypoxia.Methods Mice were randomly divided into normal control group,airtight container group,carbon monoxide group,and sodium nitrite group.A blood oxygen analyzer was used to determine the percentage content of different types of hemoglobin in mouse blood.The study was conducted from February 2021 to May 2022.Results Compared with the normal control group,mice in the airtight container group showed a significant increase in reduced hemoglobin,carbon monoxide group mice exhibited a significant increase in carboxyhemoglobin,and mice in the sodium nitrite group injected intraperitoneally showed a significant increase in high iron hemoglobin.However,the total hemoglobin difference among the four groups of mice was not statistically significant(P>0.05).Conclusion This study clarifies the quantitative relationship between observed phenomena in hypoxia model experiments by examining changes in hemoglobin properties in different types of experimentally induced hypoxia.This contributes to the improved accuracy of experimental observations.

关 键 词：缺氧 血红蛋白 还原血红蛋白 碳氧血红蛋白 高铁血红蛋白 低张性缺氧 血液性缺氧 

分 类 号：G642[文化科学—高等教育学]