基于UHPLC-Q-Exactive Orbitrap-MS的三种基原溪黄草及其干预小鼠肝纤维化的血清化学研究  

Chemical constituents in three origins of Rabdosia serra and their administration serum analysis in hepatic fibrosis mice based on UHPLC-Q-Exactive Orbitrap MS

作　　者：覃萍 苏阳静 陈永苗 郑瑞瑶 钟佳妮 叶晓燕 葛跃伟 陈阿丽[1] QIN Ping;SU Yang-jing;CHEN Yong-miao;ZHENG Rui-yao;ZHONG Jia-ni;YE Xiao-yan;GEYue-wei;CHEN A-li(Center for Drug Research and Development,Guangdong Key Laboratory of Advanced Drug Delivery System,Guangdong Pharmaceutical University,Guangzhou 510006;The First Affiliated Hospital School of Guangdong Pharmaceutical University,Guangzhou 510062;School of Chinese Material Medica,Guangdong Pharmaceutical University,Guangzhou 510006)

机构地区：[1]广东药科大学新药研发中心,广东省药物新剂型重点实验室,广州510006 [2]广东药科大学附属第一医院,广州510062 [3]广东药科大学中药学院,广州510006

出　　处：《中南药学》2024年第2期296-301,共6页Central South Pharmacy

基　　金：国家自然科学基金项目(No.82104382)。

摘　　要：目的应用超高效液相色谱-四极杆-静电场轨道阱串联质谱(UHPLC-Q-Exactive Orbitrap-MS)技术研究三种基原溪黄草及其在肝纤维化小鼠口服给药后的血清化学成分。方法采用Shim-pack GIST-HP C_(18)色谱柱(2.1 mm×150 mm,3μm);流动相为0.1%甲酸水(A)-甲醇(B),梯度洗脱,流速0.3 mL·min^(-1),柱温30℃。通过结合高分辨质谱数据及质谱裂解规律,对三种基原溪黄草药材样本及其给药小鼠血清的化学成分进行表征。结果在三种基原溪黄草药材中鉴定出40个化合物,主要包括二萜类、黄酮类、酚酸类化合物。在小鼠血清中鉴定出2个入血成分以及2个可能的代谢产物。结论本研究初步鉴定了三种基原溪黄草在四氯化碳致肝纤维化小鼠中的入血成分及部分代谢产物,为其用于治疗肝纤维化的药效物质基础提供了理论参考。Objective To identify the serum chemical compositions in three origins of Rabdosia serra and the serum in mice with hepatic fibrosis after the oral administration by UHPLC-Q-Exactive Orbitrap-MS.Methods The three origins of Rabdosia serra and the serum samples administered in mice were collected and analyzed with the chromatographic column of Shim-pack GIST-HP C_(18)(2.1 mm×150 mm,3μm).The mobile phase was 0.1%formic acid water(A)-methanol(B)for gradient elution and the flow rate was 0.3 mL·min^(-1).The column temperature was 30℃.The chemical compositions in three origins of Rabdosia serra and the serum in mice after the oral administration were analyzed with the high resolution mass spectrometry data and mass spectrometry fragmentation law of the compound.Results Totally 40 compounds were identified in the three origins of Rabdosia serra,mainly diterpenoids,flavonoids and phenolic acids.Two blood components and two possible metabolites were analyzed in the serum of mice.Conclusion This study preliminarily identifies the blood components in three origins of Rabdosia serra in mice with carbon tetrachloride induced liver fibrosis,providing a theoretical reference for the efficacy in the treatment of liver fibrosis.

关 键 词：溪黄草 肝纤维化 超高效液相色谱-四极杆-静电场轨道阱串联质谱 

分 类 号：R917.101[医药卫生—药物分析学]