灰毡毛忍冬bZIP25基因的克隆及其表达模式分析  

Cloning and expression pattern of bZIP25 transcription factor from Lonicera macranthoides

作　　者：王珊[1] 曾娟 谢瑜[1] 周日宝[2,3,4] 刘湘丹 童巧珍[2,3,4] 龙雨青 陈言[5] 刘小丽 WANG Shan;ZENG Juan;XIE Yu;ZHOU Ri-bao;LIU Xiang-dan;TONG Qiaozhen;LONG Yu-qing;CHEN Yan;LIU Xiao-Li(The Second Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410005;School of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208;Key Laboratory of Germplasm Resources and Standardized Planting of Hunan Bulk Authentic Medicinal Materials,Changsha 410208;Key Laboratory of Modern Research of Chinese Medicine,Higher Education Division epartment of Hunan,Changsha 410208;Jiaozuo Product Quality Inspection and Testing Center,Jiaozuo Henan 454000)

机构地区：[1]湖南中医药大学第二附属医院,长沙410005 [2]湖南中医药大学药学院,长沙410208 [3]湘产大宗道地药材种质资源及规范化种植重点研究室,长沙410208 [4]湖南省普通高等学校中药现代化研究重点实验室,长沙410208 [5]焦作市产品质量检验检测中心,河南焦作454000

出　　处：《中南药学》2024年第2期335-340,共6页Central South Pharmacy

基　　金：2022湖南省中医药科研计划项目(No.D2022134);2021湖南省教育厅科学研究项目(No.21C0245);2021校级科研基金与联合基金项目(No.2021XJJJ063);2020年湖南省一流专业建设点:中药资源与开发;湖南中医药大学中药学一流学科项目(校行科字【2018】3号)。

摘　　要：目的克隆灰毡毛忍冬bZIP25基因序列全长,并进行生信分析及表达模式分析,以初步探索其在灰毡毛忍冬中的生物学功能。方法通过逆转录PCR技术克隆bZIP25基因的序列全长,采用生物信息学分析方法对bZIP25及其编码的蛋白进行分析。运用实时荧光定量PCR(qRT-PCR)技术测定其在茎、叶及七个花期花中的表达水平。结果克隆得到LmbZIP25基因(OR551766),其编码191个氨基酸,具有典型的bZIP家族结构,在bZIP结构域中与其他植物同源性较高。LmbZIP25基因具有组织特异性,在茎中的表达量显著高于花和叶,在七个花期中黄色花蕾期的表达量最高。结论克隆得到LmbZIP25基因全长,分析其在不同器官和不同花期的表达差异,为进一步探索其在灰毡毛忍冬中花发育中的功能奠定了研究基础。Objective To clone the cDNA sequences of bZIP25 gene from Lonicera macranthoides,and conduct bioinformatics analysis and expression pattern analysis,to determine the biological function of bZIP25 in Lonicera macranthoides.Methods The full length of bZIP25 sequence was cloned with reverse transcription PCR technology.bZIP25 and its encoded proteins were analyzed with bioinformatics analysis methods.Its expression level in the stems,leaves and seven flowering stages were determined by real-time fluorescence quantitative PCR technology.Results The LmbZIP25(OR551766)gene was cloned,and 191 amino acids encoded.It contained a typical bZIP family structure and high homology with other plants in the bZIP domain.The LmbZIP25 gene had tissue specificity and its expression level in the stem was much higher than that in the flowers and leaves.The highest expression occurred level in the yellow bud stage among the seven flowering stages.Conclusion The full length of LmbZIP25 gene is cloned and its expression differences in organs and flowering stages are analyzed,laying a foundation for further exploring its biological functions in the floral development of Lonicera macranthoides.

关 键 词：灰毡毛忍冬 bZIP25 基因克隆 生物信息学分析 表达模式分析 

分 类 号：S567.79[农业科学—中草药栽培]