不同基原淫羊藿和巫山淫羊藿药材的化学成分差异研究  

Differences in chemical compositions of different basal herbs of epimedium and Wushan epimedium

作　　者：田祥木 李天娇[1,2,3] 李强 杨欣欣 王帅[1,2,3] 包永睿 孟宪生[1,2,3] TIAN Xiang-mu;LI Tian-jiao;LI Qiang;YANG Xin-xin;WANG Shuai;BAO Yongrui;MENG Xian-sheng(School of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian Liaoning 116600;Professional Innovation and Technology Center for Multidimensional Analysis of Traditional Chinese Medicine,Liaoning Dalian 116600;Liaoning Engineering Laboratory of Modern Traditional Chinese Medicine Research,Dalian Liaoning 116600;Hebei Baicaokang Pharmaceutical Co.,Ltd.,Hengshui Hebei 053800)

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]辽宁省中药多维分析专业技术创新中心,辽宁大连116600 [3]辽宁省现代中药研究工程实验室,辽宁大连116600 [4]河北百草康神药业有限公司,河北衡水053800

出　　处：《中南药学》2024年第2期368-375,共8页Central South Pharmacy

基　　金：辽宁省教育厅2022年度高校基本科研项目(No.LJKMZ20221318);辽宁省博士科研启动基金项目(No.2023-BS-138)。

摘　　要：目的通过UPLC-Q-TOF-MS技术,探讨不同基原淫羊藿和巫山淫羊藿的药材体外化学成分的差异,为淫羊藿及巫山淫羊藿药材鉴定及质量控制奠定基础。方法采用Agilent SBC_(18)(2.1 mm×100 mm,2.7μm)色谱柱,以0.1%甲酸水溶液-甲醇(+)及水-甲醇(-)为流动相进行梯度洗脱,流速0.4 mL·min^(-1)。质谱采用电喷雾(ESI)离子源,在正、负离子模式下进行数据采集。通过保留时间、相对分子质量及二级碎片离子对巫山淫羊藿、淫羊藿、柔毛淫羊藿、箭叶淫羊藿及朝鲜淫羊藿药材中的化学成分进行鉴定,并基于所鉴定出的化合物对不同基原淫羊藿及巫山淫羊藿药材的化学成分通过SIMCA软件进行差异分析。结果不同基原淫羊藿及巫山淫羊藿药材中共鉴定出朝霍定A、朝霍定B、朝霍定C、淫羊藿苷等37种化学成分,经过对比发现不同基原的淫羊藿及巫山淫羊藿药材中淫羊藿定L、2''-鼠李糖基淫羊藿次苷Ⅱ、木兰花碱等12种成分的相对含量具有明显差异,差异化合物经鉴定主要为黄酮类化合物。结论本试验所发现的差异化合物可以作为区分不同基原淫羊藿及巫山淫羊藿药材的指标,这5种药材的鉴别和质量控制奠定基础。Objective To determine the differences in chemical compositions of different basal herbs of epimedium and Wushan epimedium by UPLC-Q-TOF-MS,and identify and control the quality epimedium and Wushan epimedium based on their chemical compositions.Methods An Agilent SBC_18)(2.1 mm×100 mm,2.7μm)column was used with 0.1%formic acid water-methanol(+)and water-methanol(-)as the mobile phases for gradient elution at a flow rate of 0.4 mL·min^(-1).The mass spectrometry was performed with an electrospray ionization(ESI)ion source,and the data were collected in both positive and negative ion modes.The chemical components in different basal epimedium and Wushan epimedium herbs were identified via retention time,relative molecular mass and secondary fragment ions.Based on the identified compounds,the chemical compositions in the different basal epimedium and Wushan epimedium herbs were differentially analyzed by SIMCA software.Results Totally 37 chemical constituents such as epimedin A,epimedin B,epimedin C and icariin were identified in the different basal epimedium and Wushan epimedium herbs.The relative contents of 12 constituents,such as icariin L,2’-O-rhamnosylicarisideⅡand magnoflorine in the basal epimedium and Wushan epimedium herbs had obvious differences,and the differential compounds were flavonoids mainly.Conclusion The compounds found in this experiment can be used as the indicators to distinguishing different basal epimedium and Wushan epimedium,laying a foundation for the identification and quality control of the 5 kinds of epimedium herbs.

关 键 词：淫羊藿 UPLC-Q-TOF-MS 不同基原 化学成分 

分 类 号：R282.5[医药卫生—中药学] R284.1[医药卫生—中医学]