培养温度、时间对饲用凝结芽孢杆菌菌数的影响  

Effect of Culture Temperature and Time on The Number of Bacillus coagulation for Feed

作　　者：王雪艳[1] 王乙茹 黄遵锡[3] 陈德近 何雪 安清聪[1] 程志斌[1] WANG Xueyan;WANG Yiru;HUANG Zunxi;CHEN Dejin;HE Xue;AN Qingcong;CHENG Zhibin(College of Animal Science and Technology,Yunnan Agricultural University,Yunnan Kunming 650201,China;Dazhou Vocational and Technical College,Sichuan Dazhou 635001,China;School of Life Science Yunnan Normal University,Yunnan Kunming 650222,China;Qactive Bio-Engineering(Kunming)Co.,Ltd.,Yunnan Kunming 650506,China)

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]达州职业技术学院,四川达州635001 [3]云南师范大学生命科学学院,云南昆明650222 [4]爱科特生物工程(昆明)有限公司,云南昆明650506

出　　处：《饲料工业》2024年第4期127-132,共6页Feed Industry

基　　金：国家自然科学基金[31860664];云南省农业联合面上项目[2017FG001-067]。

摘　　要：研究采用平板计数法,评估了两个团体标准(T/YNBX 024—2021,简称“云南团标”;T/CSWSL 022—2020,简称“北京团标”)操作步骤中微生物的培养温度、培养时间对饲用凝结芽孢杆菌制剂的菌数检测结果的影响,旨在为确定饲用凝结芽孢杆菌制剂菌数检测的培养温度和时间提供参考。结果显示:无论采用云南团标、还是北京团标,在40℃/24 h“低温、短时”培养条件下,平板上无菌落生长,无菌数检出数据;50℃/24 h“高温、短时”培养的检出菌数显著高于40℃/48 h“低温、长时”(P<0.05),且50℃/24 h“高温、短时”与50℃/48 h“高温、长时”的检出菌数一致。本研究证明,平板计数法检测饲用凝结芽孢杆菌菌数受培养温度和时间影响,以培养温度为50℃、培养时间为24~48 h为宜。In this experiment,culture temperature and time in two existing promulgated standards T/YNBX 024-2021(Yunnan group standard)and T/CSWSL 022—2020(Beijing group standard)was evalu-ated to the aerobic count of Bacillus coagulans additive by plate count method.The aim was to provide reference for determining the cultivation temperature and time for bacterial count detection of feed Bacil-lus coagulans.The results from the determine operating procedure of both Yunnan group standard and Beijing group standard showed that no aerobic count data was detected in“Low temperature/Short time”(40℃/24 h)culture condition due to no observation of Bacillus coagulans colony.The aerobic count of Bacillus coagulans additive in“High temperature/Short time”(50℃/24 h)culture condition was more than those in“Low temperature/Long time”(40℃/48 h)culture condition.Moreover,Bacillus coagulans colony count in“High temperature/Short time”(50℃/24 h)culture condition was similar with those in“High temperature/Long time”(50℃/48 h)culture condition.These findings proved that the aerobic count of Bacillus coagulans additive by plate count method was affected by culture temperature and time,and the optimal culture condition is 50℃/24~48 h.

关 键 词：饲用凝结芽孢杆菌制剂 菌数检测 平板计数法 培养温度 培养时间 

分 类 号：S816[农业科学—饲料科学]