肝龙胶囊对Balb/c裸鼠腋下移植瘤的影响  

Effect of Ganlong capsule on axillary graft tumour in Balb/c nude mice

作　　者：杨逸鑫 王彦权 彭芳 YANG Yixin;WANG Yanquan;PENG Fang(College of Pharmacy,Dali University,Dali 671000,Yunnan,China;Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D,Dali 671000,Yunnan,China)

机构地区：[1]大理大学药学院,云南大理671000 [2]云南省昆虫生物医药研发重点实验室,云南大理671000

出　　处：《中国现代医生》2024年第4期73-76,98,共5页China Modern Doctor

摘　　要：目的探讨肝龙胶囊对Balb/c裸鼠腋下移植瘤的影响。方法将人肝癌敏感株BEL-7402接种于裸鼠腋下造模,造模成功后将其分为模型组、索拉非尼组、CII-3组、脱脂膏组、肝龙胶囊高、中、低剂量组,正常裸鼠为空白组,连续给药14d。比较各组的抑瘤率、脏器指数、生化指标、缺氧微环境相关蛋白及其mRNA表达水平。结果各药物治疗组均能抑制肿瘤生长,其中肝龙胶囊中剂量组的平均抑瘤率最高;与模型组相比,各药物治疗组裸鼠的心脏指数、肾脏指数、肝脏指数、肺脏指数均有所升高,脾脏指数均有所下降,但多数变化不大;各项生化指标显示,肝龙胶囊安全性良好,并未出现明显的组织损伤;索拉非尼组、肝龙胶囊中剂量组裸鼠的低氧诱导因子1α(hypoxia-inducible factor-1α,HIF-1α)、血管内皮生长因子(vascular endothelial growth factor,VEGF)和血管内皮生长因子受体2(vascular endothelial growth factor receptor 2,VEGFR2)的mRNA表达均显著低于模型组(P<0.05);免疫组织化学结果显示,各药物治疗组的VEGFR2、HIF-1α、VEGF、Ki-67和CD31蛋白表达均表现出不同程度的抑制,其中以索拉非尼组、肝龙胶囊中剂量组及CII-3组的抑制作用较强。结论肝龙胶囊可有效抑制Balb/c裸鼠腋下移植瘤的生长,并下调移植瘤中缺氧微环境相关蛋白及其mRNA表达水平。Objective To investigate the effect of Ganlong capsule on axillary graft tumour in Balb/c nude mice.Methods BEL-7402,a human hepatocellular carcinoma sensitive strain,was inoculated into the armpit of nude mice for modeling.After successful modeling,mice were divided into model group,sorafenib group,CII-3 group,degreasing cream group,and Ganlong capsule high-dose,medium-dose and low-dose groups,and normal nude mice were included in blank group.All groups were given continuous administration for 14 days.The tumor inhibition rate,organ index,biochemical index,anoxic microenvironment related proteins and their mRNA expression levels were compared among all groups.Results All drug treatment groups could inhibit tumor growth,and the average tumor inhibition rate was the highest in Ganlong capsule medium-dose group.Compared with the model group,the heart index,kidney index,liver index and lung index of nude mice in all drug treatment groups were increased,and the spleen index were decreased,but most of the changes were little.Various biochemical indexes showed that Ganlong capsule was safe and did not cause obvious tissue damage.The mRNA expressions of hypoxia-inducible factor-1α(HIF-1α),vascular endothelial growth factor(VEGF)and vascular endothelial growth factor receptor 2(VEGFR2)in sorafenib group and Ganlong capsule medium-dose group were significantly lower than those in model group(P<0.05).Immunohistochemical results showed that all drug treatment groups showed different degrees of inhibition on VEGFR2,HIF-1α,VEGF,Ki-67 and CD31 protein expressions,among which sorafenib group,Ganlong capsule medium-dose group and CII-3 group had stronger inhibitory effects.Conclusion Ganlong capsule can effectively inhibit the growth of axillary graft tumour in Balb/c nude mice,and down-regulate the expression level of hypoxic microenvironment related protein and their mRNA in the graft tumor.

关 键 词：肝龙胶囊 肝癌 腋下移植瘤 美洲大蠊 

分 类 号：R965.2[医药卫生—药理学]