2022年宁夏固原市肉用流产母牛病原血清学调查分析  

Serological Investigation and Analysis of Pathogens in Meat Aborted Cows in Guyuan City of Ningxia in 2022

作　　者：谢建亮 于有利 郭亚男 王景松 李珂 XIE Jian-liang;YU You-li;GUO Ya-nan;WANG Jinsong;LI Ke(Guyuan Yuanzhou District Animal Husbandry Technology Promotion Service Center,Guyuan,756000;Institute of Animal Science,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan,Ningxia,750002;School of Agriculture,Ningxia University,Yinchuan 750021)

机构地区：[1]宁夏固原市畜牧技术推广服务中心,宁夏固原756000 [2]宁夏农林科学院,宁夏银川750002 [3]宁夏大学,宁夏银川750002

出　　处：《中国牛业科学》2023年第5期77-80,共4页China Cattle Science

基　　金：宁夏回族自治区重点研发项目(2022BBF03024,2022BBF03025),宁夏回族自治区青年拔尖人才项目。

摘　　要：[目的]为了明确宁夏固原地区肉用流产母牛4种流产相关病原的流行情况。[方法]试验选择宁夏固原市5个地区不同规模化肉牛养殖场,采集131份有流产史的母牛血清样品,采用酶联免疫吸附试验(ELISA)分别对流产相关病原抗体/抗原进行检测和分析。[结果]采集的131份血清样品中共检出109份阳性血清,阳性率为83.2%。其中BVDV抗体阳性率为62.6%(82/131)、流产衣原体抗体阳性率为59.5%(78/131)、IBRV抗体阳性率为13.7%(18/131),布鲁氏菌抗体阳性率为0%(0/131)。结果表明,采集的131份血清样品中共检出109份阳性血清,阳性率为83.2%。其中BVDV抗体阳性率为62.6%(82/131)、流产衣原体抗体阳性率为59.5%(78/131)、IBRV抗体阳性率为13.7%(18/131),布鲁氏菌抗体阳性率为0%(0/131)。109份阳性样品中,最多出现3种病原混合感染。其中单一病原感染阳性样品占42.2%(46/109),以BVDV感染比例最大,与流产衣原体阳性率相比差异显著(P<0.05);2种病原混合感染样品占56.9%(62/109),以BVDV和流产衣原体混合感染比例最大,与流产衣原体和IBRV混合感染阳性率、BVDV和IBRV混合感染阳性率差异极显著(P<0.01);3种病原混合感染样本占4.6%(4/109),主要以BVDV、流产衣原体和IBRV混合感染为主。[结论]宁夏固原地区流产母牛均存在以上3种疫病感染,其中主要以BVDV和流产衣原体单独或混合感染为主。[Objective]To investigate the prevalence of 4 abortion-related pathogens in aborted cows for meat in Guyuan area of Ningxia.[Methods]131 serum samples from cows with a history of abortion were collected from 5 beef cattle farms in Guyuan City,Ningxia,and the abortion-related pathogen antibodies/antigens were detected and analyzed by enzyme-linked immunosorbent assay(ELISA).[Results]109 of 131 serum samples were found to be positive,with a positive rate of 83.2%.The positive rate of BVDV antibody was 62.6%(82/131),the positive rate of Chlamydia abortis antibody was 59.5%(78/131),the positive rate of IBRV antibody was 13.7%(18/131),and the positive rate of brucella antibody was 0%(0/131).The results showed that 109 of 131 serum samples were positive,the positive rate was 83.2%.The positive rate of BVDV antibody was 62.6%(82/131),the positive rate of Chlamydia abortis antibody was 59.5%(78/131),the positive rate of IBRV antibody was 13.7%(18/131),and the positive rate of brucella antibody was 0%(0/131).In 109 positive samples,mixed infection of up to 3 pathogens was found.Among them,42.2%(46/109)were positive for single pathogen infection,and the proportion of BVDV infection was the largest,which was significantly different from the positive rate of Chlamydia abortus(P<0.05).The proportion of mixed infection samples was 56.9%(62/109),and the proportion of mixed infection with BVDV and Chlamydia abortus was the largest,and the positive rate of mixed infection with Chlamydia abortus and IBRV,and the positive rate of BVDV and IBRV were significantly different(P<0.01).The samples of mixed infection with 3 pathogens accounted for 4.6%(4/109),mainly BVDV,Chlamydia abortus and IBRV mixed infection.[Conclusion]All aborted cows in Guyuan area of Ningxia were infected with the above three kinds of diseases,and BVDV and chlamydia abortus were mainly infected alone or mixed.

关 键 词：宁夏固原 牛病毒性腹泻病毒 流产衣原体 传染性牛鼻气管炎病毒 布鲁氏菌 抗体检测 

分 类 号：S857.22[农业科学—临床兽医学] S858.23[农业科学—兽医学]