肠道内容物和黏膜菌群对枳实导滞汤剂治疗食积便秘的响应差异  

The difference in response of intestinal contents and mucosal microbiota to treatment of dyspepsia constipation with Zhishi Daozhi decoction

作　　者：彭昕欣[1] 易鑫 刘婧 方乐瑶 蔡莹 PENG Xinxin;YI Xin;LIU Jing;FANG Leyao;CAI Ying(The First Affiliated Hospital of Hu′nan University of Chinese Medicine,Changsha,Hu′nan 410007,China;不详)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南中医药大学中医学院,湖南长沙410007

出　　处：《中国微生态学杂志》2023年第11期1241-1247,1256,共8页Chinese Journal of Microecology

基　　金：湖南省自然科学基金(2022JJ40332);湖南中医药大学中医学国内一流建设学科项目(4901-020000200207)。

摘　　要：目的 通过枳实导滞汤剂对食积便秘小鼠肠道内容物和黏膜菌群的影响差异探讨肠道菌群在枳实导滞汤剂干预食积便秘中的作用。方法 采用网络药理学预测枳实导滞汤剂缓解便秘的药物成分和潜在功能;通过高脂高蛋白饮食结合腹腔注射盐酸洛哌丁胺制备食积便秘小鼠模型,造模成功后,治疗组小鼠给予枳实导滞汤剂0.4 g/mL,正常组和模型组给予等剂量蒸馏水,每天2次,连续7 d。治疗结束后,分别收集各组小鼠肠道内容物和黏膜,通过高通量测序技术分析肠道菌群。结果 网络药理学分析表明,从枳实导滞汤剂中共获得128个潜在活性成分,并获得1 379个便秘相关的靶点,药物和便秘检测到共同靶点74个。食积便秘小鼠肠道内容物与黏膜菌群相比,ASV(扩增子序列变异)数量和多样性均较高;Beta多样性分析显示黏膜和内容物菌群差异较大。模型组内容物样本中富集Prevotella、Akkermansia、Clostridium、Jeotgalicoccus及Planococcaceae Staphylococcus,黏膜样本中富集Candidatus arthromitus、Bacillus及Acinetobacter。治疗组内容物样本中富集Bacillus,黏膜样本中富集Pseudomonas、 StaphylococcaceaeStaphylococcus。结论 食积便秘小鼠及枳实导滞汤剂干预后小鼠黏膜与内容物菌群在群落组成、多样性、特征菌等方面存在明显差异,枳实导滞汤剂干预后,肠道内容物菌群较黏膜菌群恢复更好。Objective To explore the role of intestinal flora in the intervention of Zhishi Daozhi decoction on dyspepsia constipation (DC) by analyzing the difference in the response of intestinal contents vs mucosal microbiota in mice with DC. Methods Network pharmacology was used to predict the drug composition and potential function of Zhishi Daozhi decoction in relieving constipation. The models of DC were established by combining high-fat and high-protein diet with intraperitoneal injection of loperamide hydrochloride. After the models were successfully established, the treatment group was given Zhishi Daozhi decoction 0.4 g/mL, while the normal group and the model group were given the same dose of distilled water twice a day for 7 days. After the treatment, the intestinal contents and mucosa of mice in each group were collected, and the intestinal microbiota was analyzed with high-throughput sequencing technique. Results A total of 128 potential active components were obtained from Zhishi Daozhi decoction;1,379 constipation-related targets and 74 common targets of drugs and constipation were obtained. The number and diversity of amplicon sequence variant in intestinal mucosa of mice with DC were lower than in mucosal microbiota. Beta diversity analysis showed the microbiota in mucosa and contents were quite different. The contents in the model group were enriched with Prevotella, Akkermansia, Clostridium, Jeotgalicoccus and Planococcaceae Staphylococcus, while the mucosa samples were enriched with Candidatus arthromitus, Bacillus and Acinetobacter. In the treatment group, Bacillus was enriched in the content samples, while Pseudomonas and Staphylococcae Staphylococcus were enriched in the mucosa samples. Conclusion There are obvious differences in community composition, diversity and characteristic bacteria between mucosa and contents after DC vs the intervention of Zhishi Daozhi decoction, and the microbiota in intestinal contents recovered better than that in the mucosa after the intervention of Zhishi Daozhi decoct

关 键 词：枳实导滞汤剂 食积便秘 肠道菌群 肠道内容物 肠道黏膜 

分 类 号：R289[医药卫生—方剂学]