外源钙离子对好氧活性污泥反应器溶解性有机氮特征的影响  

Effect of exogenous calcium ions on characteristics of dissolved organic nitrogen in aerobic activated sludge reactors

作　　者：游佳倩 谢琤琤 廖可薇 胡海冬 YOU Jiaqian;XIE Chengcheng;LIAO Kewei;HU Haidong(State Key Laboratory of Pollution Control and Resource Reuse,School of Environment,Nanjing University,Nanjing 210023,China;CSD New Concept Environmental Development Yixing Co.,Ltd.Yixing,China,214200)

机构地区：[1]南京大学环境学院,污染控制与资源化研究国家重点实验室,南京210023 [2]中持新概念环境发展宜兴有限公司,宜兴214200

出　　处：《环境工程学报》2023年第11期3505-3514,共10页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金面上项目(52170036);江苏省自然科学青年基金资助项目(BK20180346)。

摘　　要：为探明外源钙离子对好氧活性污泥系统溶解性有机氮(DON)的影响,考察了不同钙离子浓度下(0~1 mmol·L^(-1))出水DON浓度和生物有效性的变化,并结合傅里叶变换离子回旋共振质谱(FTICR-MS)及微生物代谢活性和群落结构分析探究钙离子影响的分子层面原因和微生物内在机理。结果表明,出水DON的浓度和生物有效性随着外源钙离子浓度的增加均呈现阈值现象,在最佳钙离子投加阈值浓度(0.1 mmol·L^(-1)Ca^(2+))下,出水DON浓度和生物有效性为1.46±0.12 mg·L^(-1)和17.97%±0.05%,比空白组(0 mmol·L^(-1)Ca^(2+))分别增加了35.2%和降低了47.6%。FTICR-MS分析DON的分子特征显示,钙离子是通过影响好氧活性污泥反应器中氧化程度较低分的组分(NOSC<0)影响了最终出水DON的生物有效性。微生物学分析表明,三磷酸腺苷、琥珀酸脱氢酶、脱氢酶、饱和脂肪酸和Luteolibacter菌是推动出水DON去除率提升和生物有效性降低的重要因素。To investigate the effect of exogenous calcium ions on dissolved organic nitrogen(DON)in aerobic activated sludge systems,this study examined the changes in effluent DON concentration and bioavailability under different calcium ion concentrations(0~1 mmol·L^(-1)).Fourier transform ion cyclotron resonance mass spectrometry(FTICR-MS)and microbial analysis of metabolic activity and commol·L^(-1)unity structure were combined to explore the molecular reasons and microbial mechanisms underlying the influence of calcium ions.The results showed that both the effluent DON concentration and bioavailability exhibited threshold phenomena with increasing exogenous calcium ion concentration,reaching 1.46±0.12 mg·L^(-1)and 17.97±0.05%at the optimal calcium ion dosage threshold(0.1 mmol·L^(-1)).At this dosage,the DON concentration and bioavailability increased by 35.2%and decreased by 47.6%compared to the blank group(0 mmol·L^(-1)),respectively.FTICR-MS analysis of DON molecular characteristics showed that calcium ions influenced the final effluent DON bioavailability mainly by affecting the less oxidative DON components(NOSC<0)in the aerobic activated sludge bioreactor.Microbiological analysis indicated that ATP,SDH,DHA,saturated fatty acids,and Luteolibacter were important factors promoting the increase in DON removal and decrease in effluent DON bioavailability.

关 键 词：钙离子 溶解性有机氮(DON) 生物有效性 傅里叶变换离子回旋共振质谱 

分 类 号：X703.1[环境科学与工程—环境工程]