变叶榕提取物不同组分总黄酮含量及抗氧化抗炎活性研究  

Total Flavonoids Contents,Antioxidant and Anti-inflammatory Activities of Different Fractions of Ficus variolosa Extraction

作　　者：吴文明 习丹[1] 邹婷婷 熊贻友 应萍[1] 胡建新[1] WU Wen-ming;XI Dan;ZOU Ting-ting;XIONG Yi-you;YING Ping;HU Jian-xin(Department of Pharmacy,Jiangxi Provincial People s Hospital(The First Affiliated Hospital of Nanchang Medical College),Jiangxi Nanchang 330006;College of Life Science and Technology of Huazhong University of Science and Technology,Hubei Wuhan 430074;Faculty of Pharmacy,Fuzhou Medical College of Nanchang University,Jiangxi Fuzhou 344000,China)

机构地区：[1]江西省人民医院(南昌医学院第一附属医院)药学部,江西南昌330006 [2]华中科技大学生命科学与技术学院,湖北武汉430074 [3]南昌大学抚州医学院药学院,江西抚州344000

出　　处：《广州化工》2023年第19期38-41,54,共5页GuangZhou Chemical Industry

基　　金：江西省自然科学基金(20192BAB215056);江西省中医药管理局科技计划重点项目(2022Z018);江西省中医药管理局中药制剂临床安全监测和转化应用重点研究室项目;江西省中医药中青年骨干人才(第二批)培养计划。

摘　　要：采用NaNO_(2)-Al(NO_(3))_(3)-NaOH法测定各组分总黄酮含量;DPPH、ABTS自由基清除及Fe^(3+)还原能力试验测定各组分抗氧化活性;Griees试剂法评价各组分抗炎活性。研究发现,变叶榕根提取物总黄酮含量为(161.26±2.74)mg/g,乙酸乙酯组分总黄酮含量最高,为(285.75±5.99)mg/g。乙酸乙酯组分对DPPH、ABTS自由基清除和Fe^(3+)还原能力的IC_(50)值分别为15.53、6.69和56.52μg/mL,对LPS诱导RAW264.7细胞释放NO的抑制活性的IC_(50)值为63.07μg/mL。结果表明,变叶榕根乙酸乙酯组分具有较明显的抗氧化抗炎活性,其化学成分及作用机制值得进一步研究。The contents of total flavonoids in variousfractions were determined by NaNO_(2)-Al(NO_(3))_(3)-NaOH assay.Antioxidant activity of the different fractions were evaluated by DPPH,ABTS free radical scavenging and reducing power measurement,and anti-inflammatory activity were evaluated by measuring NO that released from mice s abdominal macrophage RAW264.7 induced by lipopolysaccharide.The results revealed that the content of total flavonoids of ethanol extraction of Ficus variolosa roots was(161.26±2.74)μg/mL.The content of total flavonoids of ethyl acetate fraction was(285.75±5.99)μg/mL,which was significantly higher than that of any other fractions.EtOAc fraction had the most potent anti-oxidative and anti-inflammatory effect.IC 50 values for EtOAc fraction against DPPH,ABTS free radical,and reducing power were 15.53,6.69 and 56.52μg/mL,respectively.The IC_(50)values of the inhibitory activities of LPS-induced NO release in RAW264.7 cells was 63.07μg/mL.EtOAc fraction had obvious antioxidant and anti-inflammatory activities,and its chemical composition and mechanism of action deserved further study.

关 键 词：变叶榕 提取物 总黄酮 抗氧化 抗炎 

分 类 号：R932[医药卫生—生药学]